Tropin dehidrogenaza
| Tropin dehidrogenaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.1.1.206 | ||||||||
| CAS broj | 118390-87-7 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Tropin dehidrogenaza (EC 1.1.1.206, tropinon reduktaza (ambiguous), TR-I) je enzim sa sistematskim imenom tropin:NADP+ 3alfa-oksidoreduktaza.[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
- tropin + NADP+ tropinon + NADPH + H+
Tropinska dehidrogenaza takođe oksiduje druge tropan-3alfa-ole, ali ne i na korespondirajuće beta-derivate. Ovaj enzim zajedno sa EC 1.1.1.236, tropinonskom reduktazom II, predstavlja tačku grananja u tropanskom alkaloidnom metabolizmu. Tropin (produkt enzima EC 1.1.1.206 se inkorporira u hiosciamin i skopolamin, dok je pseudotropin (product enzima EC 1.1.1.236) prvi specifični metabolit na putu do kalistegina. Oba enzima su uvek zajedno prisutna u vrstama koji proizvode tropanski alkaloid. Oni imaju zajednički supstrat, tropinon, i striktno su stereospecifična.
Reference[uredi | uredi kod]
- ↑ Koelen, K.J. and Gross, G.G. (1982). „Partial purification and properties of tropine dehydrogenase from root cultures of Datura stramonium”. Planta Med. 44: 227-230.
- ↑ Couladis, M.M, Friesen, J.B., Landgrebe, M.E. and Leete, E. (1991). „Enzymes catalysing the reduction of tropinone to tropine and ψ-tropine isolated from the roots of Datura innoxia”. Pytochemistry 30: 801-805.
- ↑ Nakajima, K., Hashimoto, T. and Yamada, Y. (1993). „Two tropinone reductases with different stereospecificities are short-chain dehydrogenases evolved from a common ancestor”. Proc. Natl. Acad. Sci. USA 90: 9591-9595. PMID 8415746.
- ↑ Dräger, B. (2006). „Tropinone reductases, enzymes at the branch point of tropane alkaloid metabolism”. Phytochemistry 67: 327-337. PMID 16426652.
Literatura[uredi | uredi kod]
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.