Kob(I)irinska kiselina a,c-diamid adenoziltransferaza
| Kob(I)irinska kiselina a,c-diamid adenoziltransferaza | |||||||||
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| Identifikatori | |||||||||
| EC broj | 2.5.1.17 | ||||||||
| CAS broj | 37277-84-2 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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Kob(I)irinska kiselina a,c-diamid adenoziltransferaza (EC 2.5.1.17, CobA, CobO, ATP:korinoidna adenoziltransferaza, kob(I)alaminska adenoziltransferaza, akvakob(I)alaminska adenoziltransferaza, akvokob(I)alamin vitamin B12s adenoziltransferaza, ATP:kob(I)alamin Cobeta-adenoziltransferaza) je enzim sa sistematskim imenom ATP:kob(I)irinska kiselina-a,c-diamid Cobeta-adenoziltransferaza.[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
- (1) ATP + kob(I)irinska kiselina a,c-diamid trifosfat + adenozilkob(III)irinska kiselina a,c-diamid
- (2) ATP + kobinamid trifosfat + adenozilkobinamid
Korinoidna adenozilacija se odvija u tri stupnja: (i) redukcija Co(III) do Co(II) putem transvera jednog elektrona. To može da bude posredovano enzimom EC 1.16.1.3, akvakabalaminska reduktaza ili neenzimatski u prisustvu dihidroflavinskih nukleotida. (ii) Co(II) se redukuje do Co(I) u drugom transferu jednog elektrona posredstvom enzima EC 1.16.1.4, kob(II)alamin reduktaze i (iii) Co(I) izvodi nukleofilni napad na adenozilnu grupu ATP-a ostavljajući atom kobalta u Co(III) stanju (EC 2.5.1.17).
Reference[uredi | uredi kod]
- ↑ Vitols, E., Walker, G.A. and Huennekens, F.M. (1966). „Enzymatic conversion of vitamin B12s to a cobamide coenzyme, α-(5,6-dimethylbenzimidazolyl)deoxyadenosylcobamide (adenosyl-B12)”. J. Biol. Chem. 241: 1455-1461. PMID 5946606.
- ↑ Bauer, C.B., Fonseca, M.V., Holden, H.M., Thoden, J.B., Thompson, T.B., Escalante-Semerena, J.C. and Rayment, I. (2001). „Three-dimensional structure of ATP:corrinoid adenosyltransferase from Salmonella typhimurium in its free state, complexed with MgATP, or complexed with hydroxycobalamin and MgATP”. Biochemistry 40: 361-374. PMID 11148030.
- ↑ Fonseca, M.V. and Escalante-Semerena, J.C. (2001). „An in vitro reducing system for the enzymic conversion of cobalamin to adenosylcobalamin”. J. Biol. Chem. 276: 32101-32108. PMID 11408479.
- ↑ Suh, S. and Escalante-Semerena, J.C. (1995). „Purification and initial characterization of the ATP:corrinoid adenosyltransferase encoded by the cobA gene of Salmonella typhimurium”. J. Bacteriol. 177: 921-925. PMID 7860601.
Literatura[uredi | uredi kod]
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