Flavonoid 3',5'-hidroksilaza
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| Flavonoid 3',5'-hidroksilaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.13.88 | ||||||||
| CAS broj | 94047-23-1 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Flavonoid 3',5'-hidroksilaza (EC 1.14.13.88, flavonoidna 3',5'-hidroksilaza) je enzim sa sistematskim imenom flavanon,NADPH:kiseonik oksidoreduktaza.[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
- flavanon + 2 NADPH + 2 H+ + 2 O2 3',5'-dihidroksiflavanon + 2 NADP+ + 2H2O (sveukupna reakcija)
- (1a) flavanon + NADPH + H+ + O2 3'-hidroksiflavanon + NADP+ + H2O
- (1b) 3'-hidroksiflavanon + NADPH + H+ + O2 3',5'-dihidroksiflavanon + NADP+ + H2O
Ovaj enzim je hem-tiolatni protein (P-450). 3',5'-dihidroksiflavanon se formira putem 3'-hidroksiflavanona.
Reference[uredi | uredi kod]
- ↑ Menting, J., Scopes, R.K. and Stevenson, T.W. (1994). „Characterization of flavonoid 3′,5′-hydroxylase in microsomal membrane fraction of Petunia hybrida flowers”. Plant Physiol. 106: 633-642. PMID 12232356.
- ↑ Shimada, Y., Nakano-Shimada, R., Ohbayashi, M., Okinaka, Y., Kiyokawa, S. and Kikuchi, Y. (1999). „Expression of chimeric P450 genes encoding flavonoid-3′, 5′-hydroxylase in transgenic tobacco and petunia plants1”. FEBS Lett. 461: 241-245. PMID 10567704.
- ↑ de Vetten, N., ter Horst, J., van Schaik, H.P., de Boer, A., Mol, J. and Koes, R. (1999). „A cytochrome b5 is required for full activity of flavonoid 3′, 5′-hydroxylase, a cytochrome P450 involved in the formation of blue flower colors”. Proc. Natl. Acad. Sci. USA 96: 778-783. PMID 9892710.
Literatura[uredi | uredi kod]
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.