Cijanoficinaza
| Cijanoficinaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 3.4.15.6 | ||||||||
| CAS broj | 131554-16-0 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Cijanoficinaza (EC 3.4.15.6, cijanoficin degradirajući enzim, beta-Asp-Arg hidrolizujući enzim, CGPaza, CphB, CphE, cijanoficin granularna polipeptidaza, ekstracelularna CGPaza) je enzim.[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
- [L-Asp(4-L-Arg)]n + H2O [L-Asp(4-L-Arg)]n-1 + L-Asp(4--L-Arg)
Ovaj enzim je visoko specifičan za razgranate polipeptidne cijanoficine i ne hidrolizuje poli-L-aspartat ili poli-L-arginin.
- ↑ Obst, M., Krug, A., Luftmann, H. and Steinbüchel, A. (2005). „Degradation of cyanophycin by Sedimentibacter hongkongensis strain KI and Citrobacter amalonaticus strain G isolated from an anaerobic bacterial consortium”. Appl. Environ. Microbiol. 71: 3642-3652. PMID 16000772.
- ↑ Obst, M., Oppermann-Sanio, F.B., Luftmann, H. and Steinbüchel, A. (2002). „Isolation of cyanophycin-degrading bacteria, cloning and characterization of an extracellular cyanophycinase gene (cphE) from Pseudomonas anguilliseptica strain BI. The cphE gene from P. anguilliseptica BI encodes a cyanophycin-hydrolyzing enzyme”. J. Biol. Chem. 277: 25096-25105. PMID 11986309.
- ↑ Richter, R., Hejazi, M., Kraft, R., Ziegler, K. and Lockau, W. (1999). „Cyanophycinase, a peptidase degrading the cyanobacterial reserve material multi-L-arginyl-poly-L-aspartic acid (cyanophycin): molecular cloning of the gene of Synechocystis sp. PCC 6803, expression in Escherichia coli, and biochemical characterization of the purified enzyme”. Eur. J. Biochem. 263: 163-169. PMID 10429200.
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