23S rRNK (adenin2503-C2,C8)-dimetiltransferaza
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| 23S rRNK (adenin2503-C2,C8)-dimetiltransferaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.1.1.194 | ||||||||
| CAS broj | 39369-30-7 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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23S rRNK (adenin2503-C2,C8)-dimetiltransferaza (EC 2.1.1.194, Cfr, Cfr metiltransferaza, Cfr rRNK metiltransferaza) je enzim sa sistematskim imenom S-adenozil-L-metionin:23S rRNK (adenin2503-C2,C8)-dimetiltransferaza.[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
- 2 S-adenozil-L-metionin + adenin2503 u 23S rRNK 2 S-adenozil-L-homocistein + 2,8-dimetiladenin2503 u 23S rRNK
Ovaj enzim sadrži [4Fe-S] klaster. Cfr je plazmidom unesena metiltransferaza koja štiti ćelije od dejstva antibiotika. Cfr metiliše poziciju 2 ostatka A2503 nakon što je primarna metilacija u poziciji 8 završena, cf. 23S rRNK (adenin2503-C2)-metiltransferaza (EC 2.1.1.192).
Reference[uredi | uredi kod]
- ↑ Yan, F., LaMarre, J.M., Röhrich, R., Wiesner, J., Jomaa, H., Mankin, A.S., Fujimori, D.G. (2010). „RlmN and Cfr are radical SAM enzymes involved in methylation of ribosomal RNA”. J. Am. Chem. Soc. 132: 3953-3964. PMID 20184321.
- ↑ Giessing, A.M., Jensen, S.S., Rasmussen, A., Hansen, L.H., Gondela, A., Long, K., Vester, B. and Kirpekar, F. (2009). „Identification of 8-methyladenosine as the modification catalyzed by the radical SAM methyltransferase Cfr that confers antibiotic resistance in bacteria”. RNA 15: 327-336. PMID 19144912.
- ↑ Kaminska, K.H., Purta, E., Hansen, L.H., Bujnicki, J.M., Vester, B. and Long, K.S. (2010). „Insights into the structure, function and evolution of the radical-SAM 23S rRNA methyltransferase Cfr that confers antibiotic resistance in bacteria”. Nucleic Acids Res. 38: 1652-1663. PMID 20007606.
Literatura[uredi | uredi kod]
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.