Izoleucin N-monooksigenaza
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| Izoleucin N-monooksigenaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.13.117 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Izoleucin N-monooksigenaza (EC 1.14.13.117, CYP79D3, CYP79D4) je enzim sa sistematskim imenom L-izoleucin,NADPH:kiseonik oksidoreduktaza (N-hidroksilacija).[1][2] Ovaj enzim katalizuje sledeću hemijsku reakciju
- L-isoleucin + 2 O2 + 2 NADPH + 2 H+ (E)-2-metilbutanal oksim + 2 NADP+ + CO2 + 3H2O (sveukupna reakcija)
- (1a) L-isoleucin + O2 + NADPH + H+ N-hidroksi-L-isoleucin + NADP+ +H2O
- (1b) N-hidroksi-L-isoleucin + O2 + NADPH + H+ N,N-dihidroksi-L-isoleucin + NADP+ +H2O
- (1c) N,N-dihidroksi-L-isoleucin (E)-2-metilbutanal oksim + CO2 +H2O (spontaneous)
Ovaj enzim je heme-tiolatni protein (P-450). On katalizuje dve sukcesive N-hidroksilacije L-izoleucina.
Reference[uredi | uredi kod]
- ↑ Andersen, M.D., Busk, P.K., Svendsen, I. and Moller, B.L. (2000). „Cytochromes P-450 from cassava (Manihot esculenta Crantz) catalyzing the first steps in the biosynthesis of the cyanogenic glucosides linamarin and lotaustralin. Cloning, functional expression in Pichia pastoris, and substrate specificity of the isolated recombinant enzymes”. J. Biol. Chem. 275: 1966-1975. PMID 10636899.
- ↑ Forslund, K., Morant, M., Jorgensen, B., Olsen, C.E., Asamizu, E., Sato, S., Tabata, S. and Bak, S. (2004). „Biosynthesis of the nitrile glucosides rhodiocyanoside A and D and the cyanogenic glucosides lotaustralin and linamarin in Lotus japonicus”. Plant Physiol. 135: 71-84. PMID 15122013.
Literatura[uredi | uredi kod]
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.