2-Metoksi-6-poliprenil-1,4-benzohinol metilaza
| 2-Metoksi-6-poliprenil-1,4-benzohinol metilaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.1.1.201 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
2-Metoksi-6-poliprenil-1,4-benzohinol metilaza (EC 2.1.1.201, ubiE (gen, nespecifična)) je enzim sa sistematskim imenom S-adenozil-L-metionin:2-metoksi-6-sve-trans-poliprenil-1,4-benzohinol 5-C-metiltransferaza.[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
- S-adenozil-L-metionin + 2-metoksi-6-sve-trans-poliprenil-1,4-benzohinol S-adenozil-L-homocistein + 6-metoksi-3-metil-2-sve-trans-poliprenil-1,4-benzohinol
Ovaj enzim učestvuje u biosintezi ubihinona. Ubihinoni iz različitih organizama imaju različite brojeve prenilnih jedinica (na primer, ubihinon-6 kod Saccharomyces, ubihinon-9 kod pacova i ubihinon-10 i ljudi), i stoga prirodni supstrat enzima iz različitih organizama ima različite brojeve prenilnih jedinica. Enzim obično ima nisku specifičnost u pogledu broja prenilnih jedinica. Na primer, kad se COQ5 gen iz Saccharomyces cerevisiae uvede u Escherichia coli, on koriguje respiratornu deficijenciju ubiE mutanta. Bifunkcionalni enzim iz Escherichia coli takođe katalizuje metilaciju demetilmenahinola-8 (ta aktivnost se klasifikuje kao EC 2.1.1.163).
- ↑ Lee, P.T., Hsu, A.Y., Ha, H.T. and Clarke, C.F. (1997). „A C-methyltransferase involved in both ubiquinone and menaquinone biosynthesis: isolation and identification of the Escherichia coli ubiE gene”. J. Bacteriol. 179: 1748-1754. PMID 9045837.
- ↑ Young, I.G., McCann, L.M., Stroobant, P. and Gibson, F. (1971). „Characterization and genetic analysis of mutant strains of Escherichia coli K-12 accumulating the biquinone precursors 2-octaprenyl-6-methoxy-1,4-benzoquinone and 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone”. J. Bacteriol. 105: 769-778. PMID 4323297.
- ↑ Dibrov, E., Robinson, K.M. and Lemire, B.D. (1997). „The COQ5 gene encodes a yeast mitochondrial protein necessary for ubiquinone biosynthesis and the assembly of the respiratory chain”. J. Biol. Chem. 272: 9175-9181. PMID 9083048.
- ↑ Barkovich, R.J., Shtanko, A., Shepherd, J.A., Lee, P.T., Myles, D.C., Tzagoloff, A. and Clarke, C.F. (1997). „Characterization of the COQ5 gene from Saccharomyces cerevisiae. Evidence for a C-methyltransferase in ubiquinone biosynthesis”. J. Biol. Chem. 272: 9182-9188. PMID 9083049.
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