Restrikcioni enzim
Restrikcioni enzim (restrikciona endonukleaza) je enzim koji preseca DNK na specifičnim prepoznatljivim nukleotidnim sekvencama poznatim kao restrikciona mesta. Tip II restrikcioni enzimi presecaju dvolančanu DNK.[1][2][3] Ovi enzimi, nađeni kod bakterija i arheja, su verovatno nastali kao odbrambeni mehanizam protiv virusne invazije.[4][5] Unutar bakterije, restricioni enzimi selektivno presecaju stranu DNK u procesu restrikcije. DNK domaćina je metilisana putem modifikacionog enzima (metilaze) da bi se zaštitila od dejstva restrikcionih enzima. Kolektivno, ta dva procesa formiraju restrikcioni modifikacioni sistem.[6] Da bi presekao DNK, restrikcioni enzim pravi dva zaseka, jednom kroz svaki šećerno-fosfatni lanac DNK dvostrukog heliksa.
Preko 3000 restrikcionih enzima je detaljno ispitano, i više od 600 je komercijalno dostupno[7] i rutinski se koristi za DNK modifikaciju i manipulaciu u laboratorijama.[8][9][10]
Vidi još[uredi | uredi kod]
Reference[uredi | uredi kod]
- ↑ Roberts RJ; Murray, Kenneth (November 1976). „Restriction endonucleases”. CRC Crit. Rev. Biochem. 4 (2): 123–64. DOI:10.3109/10409237609105456. PMID 795607.
- ↑ Kessler C, Manta V (August 1990). „Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3)”. Gene 92 (1–2): 1–248. DOI:10.1016/0378-1119(90)90486-B. PMID 2172084.
- ↑ Pingoud A, Alves J, Geiger R (1993). „Chapter 8: Restriction Enzymes”. u: Burrell, Michael. Enzymes of Molecular Biology. Methods of Molecular Biology. 16. Totowa, NJ: Humana Press. str. 107–200. ISBN 0-89603-234-5.
- ↑ Arber W, Linn S (1969). „DNA modification and restriction”. Annu. Rev. Biochem. 38: 467–500. DOI:10.1146/annurev.bi.38.070169.002343. PMID 4897066.
- ↑ Krüger DH, Bickle TA (September 1983). „Bacteriophage survival: multiple mechanisms for avoiding the deoxyribonucleic acid restriction systems of their hosts”. Microbiol. Rev. 47 (3): 345–60. PMC 281580. PMID 6314109.
- ↑ Kobayashi I (September 2001). „Behavior of restriction–modification systems as selfish mobile elements and their impact on genome evolution”. Nucleic Acids Res. 29 (18): 3742–56. DOI:10.1093/nar/29.18.3742. PMC 55917. PMID 11557807.
- ↑ Roberts RJ, Vincze T, Posfai J, Macelis D. (2007). „REBASE—enzymes and genes for DNA restriction and modification”. Nucleic Acids Res 35 (Database issue): D269–70. DOI:10.1093/nar/gkl891. PMC 1899104. PMID 17202163.
- ↑ Primrose, Sandy B.; Old, R. W. (1994). Principles of gene manipulation: an introduction to genetic engineering. Oxford: Blackwell Scientific. ISBN 0-632-03712-1.
- ↑ Micklos, David A.; Bloom, Mark V.; Freyer, Greg A. (1996). Laboratory DNA science: an introduction to recombinant DNA techniques and methods of genome analysis. Menlo Park, Calif: Benjamin/Cummings Pub. Co. ISBN 0-8053-3040-2.
- ↑ Adrianne Massey; Helen Kreuzer (2001). Recombinant DNA and Biotechnology: A Guide for Students. Washington, D.C: ASM Press. ISBN 1-55581-176-0.
Literatura[uredi | uredi kod]
- Firman K (24. 11. 2007.). „Type I Restriction-Modification”. University of Portsmouth. Arhivirano iz originala na datum 2008-07-06. Pristupljeno 6. 6. 2008.
- Goodsell DS (1. 8. 2000.). „Restriction Enzymes”. Molecule of the Month. RCSB Protein Data Bank. Arhivirano iz originala na datum 2009-07-05. Pristupljeno 6. 6. 2008.
- Simmer M, Secko D (1. 8. 2003.). „Restriction Endonucleases: Molecular Scissors for Specifically Cutting DNA”. The Science Creative Quarterly. Pristupljeno 6. 6. 2008.
- Pingoud A, Alves J, Geiger R (1993). „Chapter 8: Restriction Enzymes”. u: Burrell, Michael. Enzymes of Molecular Biology. Methods of Molecular Biology. 16. Totowa, NJ: Humana Press. str. 107–200.
- Primrose, Sandy B.; Old, R.. W. (1994). Principles of gene manipulation: an introduction to genetic engineering. Oxford: Blackwell Scientific.
- Micklos, 2 Mark V.; Bloom; Freyer (1996). Laboratory DNA science: an introduction to recombinant DNA techniques and methods of genome analysis. Menlo Park, Calif: Benjamin/Cummings Pub. Co.
- Adrianne Massey; Helen Kreuzer (2001). Recombinant DNA and Biotechnology: A Guide for Students. Washington, D.C: ASM Press.
Spoljašnje veze[uredi | uredi kod]
- MeSH DNA Restrikcioni enzimi
- Bikandi J, San Millán R, Rementeria A, and Garaizar J. „Restriction enzyme digest of DNA”. insilico.ehu.es. Pristupljeno 28-05-2012.