Metanol dehidrogenaza (nikotinoprotein)
(Preusmjereno sa stranice NDMA-dependent methanol dehydrogenase)
| Metanol dehidrogenaza (nikotinoprotein) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.1.99.37 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Metanol dehidrogenaza (nikotinoprotein) (EC 1.1.99.37, NDMA-zavisna metanolna dehidrogenaza, nikotinoprotein metanolna dehidrogenaza, metanol:N,N-dimetil-4-nitrosoaniline oksidoreduktaza) je enzim sa sistematskim imenom metanol:akceptor oksidoreduktaza.[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju
- metanol + akceptor formaldehid + redukovani akceptor
Ovaj enzim sadrži Zn2+ i Mg2+. Nikotinoprotein metanolne dehidrogenaze sadrže čvrsto vezani NADP+/NADPH kofaktor koji se ne disocira tokom katalitičkog procesa. Umesto toga kofaktor se regeneratiše drugim supstratom ili nosiocem elektrona.
Reference[uredi | uredi kod]
- ↑ Vonck, J., Arfman, N., De Vries, G.E., Van Beeumen, J., Van Bruggen, E.F. and Dijkhuizen, L. (1991). „Electron microscopic analysis and biochemical characterization of a novel methanol dehydrogenase from the thermotolerant Bacillus sp. C1”. J. Biol. Chem. 266: 3949-3954. PMID 1995642.
- ↑ Van Ophem, P.W., Van Beeumen, J. and Duine, J.A. (1993). „Nicotinoprotein [NAD(P)-containing] alcohol/aldehyde oxidoreductases. Purification and characterization of a novel type from Amycolatopsis methanolica”. Eur. J. Biochem. 212: 819-826. PMID 8385013.
- ↑ Bystrykh, L.V., Vonck, J., van Bruggen, E.F., van Beeumen, J., Samyn, B., Govorukhina, N.I., Arfman, N., Duine, J.A. and Dijkhuizen, L. (1993). „Electron microscopic analysis and structural characterization of novel NADP(H)-containing methanol: N,N′-dimethyl-4-nitrosoaniline oxidoreductases from the gram-positive methylotrophic bacteria Amycolatopsis methanolica and Mycobacterium gastri MB19”. J. Bacteriol. 175: 1814-1822. PMID 8449887.
- ↑ Hektor, H.J., Kloosterman, H. and Dijkhuizen, L. (2002). „Identification of a magnesium-dependent NAD(P)(H)-binding domain in the nicotinoprotein methanol dehydrogenase from Bacillus methanolicus”. J. Biol. Chem. 277: 46966-46973. PMID 12351635.
- ↑ Park, H., Lee, H., Ro, Y.T. and Kim, Y.M. (2010). „Identification and functional characterization of a gene for the methanol : N,N′-dimethyl-4-nitrosoaniline oxidoreductase from Mycobacterium sp. strain JC1 (DSM 3803)”. Microbiology 156: 463-471. PMID 19875438.
Literatura[uredi | uredi kod]
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.