3-(3-hidroksifenil)propanoat hidroksilaza
(Preusmjereno sa stranice MhpA (gen))
3-(3-hidroksifenil)propanoat hidroksilaza | |||||||||
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Identifikatori | |||||||||
EC broj | 1.14.13.127 | ||||||||
IntEnz | IntEnz view | ||||||||
BRENDA | BRENDA entry | ||||||||
ExPASy | NiceZyme view | ||||||||
KEGG | KEGG entry | ||||||||
MetaCyc | metabolic pathway | ||||||||
PRIAM | profile | ||||||||
PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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3-(3-hidroksifenil)propanoat hidroksilaza (EC 1.14.13.127, mhpA (gen)) je enzim sa sistematskim imenom 3-(3-hidroksifenil)propanoat,NADH:kiseonik oksidoreduktaza (2-hidroksilacija).[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
- (1) 3-(3-hidroksifenil)propanoat + NADH + H+ + O2 3-(2,3-dihidroksifenil)propanoat + H2O + NAD+
- (2) (2E)-3-(3-hidroksifenil)prop-2-enoat + NADH + H+ + O2 (2E)-3-(2,3-dihidroksifenil)prop-2-enoat + H2O + NAD+
Ovaj enzim je flavoprotein (FAD). On učestvuje u meta odvanju kod bakterije Escherichia coli pri degradaciji raznih fenilpropanoidnih jedinjenja.
Reference[uredi | uredi kod]
- ↑ Burlingame, R. and Chapman, P.J. (1983). „Catabolism of phenylpropionic acid and its 3-hydroxy derivative by Escherichia coli”. J. Bacteriol. 155: 113-121. PMID 6345502.
- ↑ Burlingame, R.P., Wyman, L. and Chapman, P.J. (1986). „Isolation and characterization of Escherichia coli mutants defective for phenylpropionate degradation”. J. Bacteriol. 168: 55-64. PMID 3531186.
- ↑ Ferrández, A., García, J.L. and Díaz, E. (1997). „Genetic characterization and expression in heterologous hosts of the 3-(3-hydroxyphenyl)propionate catabolic pathway of Escherichia coli K-12”. J. Bacteriol. 179: 2573-2581. PMID 9098055.
- ↑ Díaz, E., Ferrández, A. and García, J.L. (1998). „Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12”. J. Bacteriol. 180: 2915-2923. PMID 9603882.
Literatura[uredi | uredi kod]
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.