D-Man-alfa-(1-3)-D-Glc-beta-(1-4)-D-Glc-alfa-1-difosfoundekaprenol 2-beta-glukuroniltransferaza
(Preusmjereno sa stranice D-Man-alfa-(1-3)-D-Glc-beta-(1-4)-D-Glc-alfa-1-difosfoundekaprenolna 2-beta-glukuroniltransferaza)
| D-Man-alfa-(1->3)-D-Glc-beta-(1->4)-D-Glc-alfa-1-difosfoundekaprenol 2-beta-glukuroniltransferaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.4.1.264 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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D-Man-alfa-(1->3)-D-Glc-beta-(1->4)-D-Glc-alfa-1-difosfoundekaprenol 2-beta-glukuroniltransferaza (EC 2.4.1.264, GumK) je enzim sa sistematskim imenom UDP-glukuronat:D-Man-alfa-(1->3)-D-Glc-beta-(1->4)-D-Glc-alfa-1-difosfo-ditrans,oktacis-undekaprenol beta-1,2-glukuroniltransferaza.[1][2][3][4][5][6][7] Ovaj enzim katalizuje sledeću hemijsku reakciju
- UDP-glukuronat + D-Man-alfa-(1->3)-D-Glc-beta-(1->4)-D-Glc-alfa-1-difosfo-ditrans,oktacis-undekaprenol UDP + D-GlcA-beta-(1->2)-D-Man-alfa-(1->3)-D-Glc-beta-(1->4)-D-Glc-alfa-1-difosfo-ditrans,oktacis-undekaprenol
Ovaj enzim učestvuje u biosintezi eksopolisaharida ksantana (kod bakterije Xanthomonas campestris) i acetana (kod bakterije Glukonacetobacter xylinus).
- ↑ Katzen, F., Ferreiro, D.U., Oddo, C.G., Ielmini, M.V., Becker, A., Puhler, A. and Ielpi, L. (1998). „Xanthomonas campestris' pv. campestris gum mutants: effects on xanthan biosynthesis and plant virulence”. J. Bacteriol. 180: 1607-1617. PMID 9537354.
- ↑ Ielpi, L., Couso, R.O. and Dankert, M.A. (1993). „Sequential assembly and polymerization of the polyprenol-linked pentasaccharide repeating unit of the xanthan polysaccharide in Xanthomonas campestris”. J. Bacteriol. 175: 2490-2500. PMID 7683019.
- ↑ Kim, S.Y., Kim, J.G., Lee, B.M. and Cho, J.Y. (2009). „Mutational analysis of the gum gene cluster required for xanthan biosynthesis in Xanthomonas oryzae' pv oryzae”. Biotechnol. Lett. 31: 265-270. PMID 18854951.
- ↑ Barreras, M., Bianchet, M.A. and Ielpi, L. (2006). „Crystallization and preliminary crystallographic characterization of GumK, a membrane-associated glucuronosyltransferase from Xanthomonas campestris required for xanthan polysaccharide synthesis”. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 62: 880-883. PMID 16946469.
- ↑ Barreras, M., Salinas, S.R., Abdian, P.L., Kampel, M.A. and Ielpi, L. (2008). „Structure and mechanism of GumK, a membrane-associated glucuronosyltransferase”. J. Biol. Chem. 283: 25027-25035. PMID 18596046.
- ↑ Vojnov, A.A., Bassi, D.E., Daniels, M.J. and Dankert, M.A. (2002). „Biosynthesis of a substituted cellulose from a mutant strain of Xanthomonas campestris”. Carbohydr. Res. 337: 315-326. PMID 11841812.
- ↑ Barreras, M., Abdian, P.L. and Ielpi, L. (2004). „Functional characterization of GumK, a membrane-associated β-glucuronosyltransferase from Xanthomonas campestris required for xanthan polysaccharide synthesis”. Glycobiology 14: 233-241. PMID 14736729.
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