4,4'-Diapofitoen sintaza
(Preusmjereno sa stranice 4,4'-diapofitoen sintaza)
| 4,4'-Diapofitoen sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.5.1.96 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
4,4'-Diapofitoen sintaza (EC 2.5.1.96, dehidroskvalenska sintaza, DAP sintaza, C30 karotenska sintaza, CrtM) je enzim sa sistematskim imenom farnezil-difosfat:farnezil-difosfat farneziltransferaza (formira 4,4'-diapofitoen).[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
- 2 (2E,6E)-farnezil difosfat 4,4'-diapofitoen + 2 difosfat (sveukupna reakcija)
- (1a) 2 (2E,6E)-farnezil difosfat difosfat + preskvalen difosfat
- (1b) preskvalen difosfat 4,4'-diapofitoen + difosfat
Za dejstvo ovog enzima je neophodan jon Mn2+. On se tipično javlja kod Staphylococcus aureus i nekih drugih bakterija, npr. Heliobacillus sp.
Reference[uredi | uredi kod]
- ↑ Umeno, D., Tobias, A.V. and Arnold, F.H. (2002). „Evolution of the C30 carotenoid synthase CrtM for function in a C40 pathway”. J. Bacteriol. 184: 6690-6699. PMID 12426357.
- ↑ Pelz, A., Wieland, K.P., Putzbach, K., Hentschel, P., Albert, K. and Gotz, F. (2005). „Structure and biosynthesis of staphyloxanthin from Staphylococcus aureus”. J. Biol. Chem. 280: 32493-32498. PMID 16020541.
- ↑ Ku, B., Jeong, J.C., Mijts, B.N., Schmidt-Dannert, C. and Dordick, J.S. (2005). „Preparation, characterization, and optimization of an in vitro C30 carotenoid pathway”. Appl. Environ. Microbiol. 71: 6578-6583. PMID 16269684.
- ↑ Liu, C.I., Liu, G.Y., Song, Y., Yin, F., Hensler, M.E., Jeng, W.Y., Nizet, V., Wang, A.H. and Oldfield, E. (2008). „A cholesterol biosynthesis inhibitor blocks Staphylococcus aureus virulence”. Science 319: 1391-1394. PMID 18276850.
Literatura[uredi | uredi kod]
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.