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[[Datoteka:Aptamer biotin.png|thumb|Struktura RNK aptamera specifičnog za [[biotin]]. Površina i osnova aptamera su prikazani žuto. Biotin (sfere) se dobro uklapa u šupljinu RNK površine]] |
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'''Aptameri''' (od {{jez-lat-lat|aptus}} - stati, i {{jez-grč-lat|meros}} - region) su [[oligonukleotid|oligonukleinske]] kiseline ili [[peptid]]ni molekuli koji se vezuju za specifični ciljni molekul. Prirodni aptameri postoje kao [[riboprekidač]]i. U laboratoriji se aptameri opično formiraju selekcijom iz velikog mnoštva randomnih sekvenci. Aptameri nalaze primenu u baznim istraživanjima, a koriste se i za kliničke svrhe kao makromolekulski lekovi. Aptameri se mogu kombinovati sa [[ribozim]]ima radi samostalnog presecanja u prisustvu ciljnog molekula. |
'''Aptameri''' (od {{jez-lat-lat|aptus}} - stati, i {{jez-grč-lat|meros}} - region) su [[oligonukleotid|oligonukleinske]] kiseline ili [[peptid]]ni molekuli koji se vezuju za specifični ciljni molekul. Prirodni aptameri postoje kao [[riboprekidač]]i. U laboratoriji se aptameri opično formiraju selekcijom iz velikog mnoštva randomnih sekvenci. Aptameri nalaze primenu u baznim istraživanjima, a koriste se i za kliničke svrhe kao makromolekulski lekovi. Aptameri se mogu kombinovati sa [[ribozim]]ima radi samostalnog presecanja u prisustvu ciljnog molekula. |
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== Literatura == |
== Literatura == |
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*{{cite journal |author=Ellington AD, Szostak JW |title=In vitro selection of RNA molecules that bind specific ligands |journal=Nature |volume=346 |issue=6287 |pages=818–22 |year=1990 |month=Aug |pmid=1697402 |doi=10.1038/346818a0 }} |
* {{cite journal |author=Ellington AD, Szostak JW |title=In vitro selection of RNA molecules that bind specific ligands |journal=Nature |volume=346 |issue=6287 |pages=818–22 |year=1990 |month=Aug |pmid=1697402 |doi=10.1038/346818a0 }} |
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*{{cite journal |author=Bock LC, Griffin LC, Latham JA, Vermaas EH, Toole JJ |title=Selection of single-stranded DNA molecules that bind and inhibit human thrombin |journal=Nature |volume=355 |issue=6360 |pages=564–6 |year=1992 |month=Feb |pmid=1741036 |doi=10.1038/355564a0 }} |
* {{cite journal |author=Bock LC, Griffin LC, Latham JA, Vermaas EH, Toole JJ |title=Selection of single-stranded DNA molecules that bind and inhibit human thrombin |journal=Nature |volume=355 |issue=6360 |pages=564–6 |year=1992 |month=Feb |pmid=1741036 |doi=10.1038/355564a0 }} |
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*{{cite journal |author=Hoppe-Seyler F, Butz K |title=Peptide aptamers: powerful new tools for molecular medicine |journal=J Mol Med. |volume=78 |issue=8 |pages=426–30 |year=2000 |pmid=11097111 |url=http://link.springer.de/link/service/journals/00109/bibs/0078008/00780426.htm |doi=10.1007/s001090000140}} |
* {{cite journal |author=Hoppe-Seyler F, Butz K |title=Peptide aptamers: powerful new tools for molecular medicine |journal=J Mol Med. |volume=78 |issue=8 |pages=426–30 |year=2000 |pmid=11097111 |url=http://link.springer.de/link/service/journals/00109/bibs/0078008/00780426.htm |doi=10.1007/s001090000140}} |
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*{{cite journal |author=Carothers JM, Oestreich SC, Davis JH, Szostak JW |title=Informational complexity and functional activity of RNA structures |journal=J Am Chem Soc. |volume=126 |issue=16 |pages=5130–7 |year=2004 |month=Apr |pmid=15099096 |doi=10.1021/ja031504a }} |
* {{cite journal |author=Carothers JM, Oestreich SC, Davis JH, Szostak JW |title=Informational complexity and functional activity of RNA structures |journal=J Am Chem Soc. |volume=126 |issue=16 |pages=5130–7 |year=2004 |month=Apr |pmid=15099096 |doi=10.1021/ja031504a }} |
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*{{cite journal |author=Cohen BA, Colas P, Brent R |title=An artificial cell-cycle inhibitor isolated from a combinatorial library |journal=Proc Natl Acad Sci USA. |volume=95 |issue=24 |pages=14272–7 |year=1998 |month=Nov |pmid=9826690 |pmc=24363 |url=http://www.pnas.org/cgi/pmidlookup?view=long&pmid=9826690 |doi=10.1073/pnas.95.24.14272}} |
* {{cite journal |author=Cohen BA, Colas P, Brent R |title=An artificial cell-cycle inhibitor isolated from a combinatorial library |journal=Proc Natl Acad Sci USA. |volume=95 |issue=24 |pages=14272–7 |year=1998 |month=Nov |pmid=9826690 |pmc=24363 |url=http://www.pnas.org/cgi/pmidlookup?view=long&pmid=9826690 |doi=10.1073/pnas.95.24.14272}} |
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*{{cite journal |author=Binkowski BF, Miller RA, Belshaw PJ |title=Ligand-regulated peptides: a general approach for modulating protein-peptide interactions with small molecules |journal=Chem Biol. |volume=12 |issue=7 |pages=847–55 |year=2005 |month=Jul |pmid=16039531 |doi=10.1016/j.chembiol.2005.05.021 }} |
* {{cite journal |author=Binkowski BF, Miller RA, Belshaw PJ |title=Ligand-regulated peptides: a general approach for modulating protein-peptide interactions with small molecules |journal=Chem Biol. |volume=12 |issue=7 |pages=847–55 |year=2005 |month=Jul |pmid=16039531 |doi=10.1016/j.chembiol.2005.05.021 }} |
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*{{cite journal |author=Sullenger BA, Gilboa E |title=Emerging clinical applications of RNA |journal=Nature |volume=418 |issue=6894 |pages=252–8 |year=2002 |month=Jul |pmid=12110902 |doi=10.1038/418252a }} |
* {{cite journal |author=Sullenger BA, Gilboa E |title=Emerging clinical applications of RNA |journal=Nature |volume=418 |issue=6894 |pages=252–8 |year=2002 |month=Jul |pmid=12110902 |doi=10.1038/418252a }} |
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*{{cite journal |author=Ng EW, Shima DT, Calias P, Cunningham ET, Guyer DR, Adamis AP |title=Pegaptanib, a targeted anti-VEGF aptamer for ocular vascular disease |journal=Nat Rev Drug Discov |volume=5 |issue=2 |pages=123–32 |year=2006 |month=Feb |pmid=16518379 |doi=10.1038/nrd1955 }} |
* {{cite journal |author=Ng EW, Shima DT, Calias P, Cunningham ET, Guyer DR, Adamis AP |title=Pegaptanib, a targeted anti-VEGF aptamer for ocular vascular disease |journal=Nat Rev Drug Discov |volume=5 |issue=2 |pages=123–32 |year=2006 |month=Feb |pmid=16518379 |doi=10.1038/nrd1955 }} |
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*{{cite journal |author=Drabovich AP, Berezovski M, Okhonin V, Krylov SN |title=Selection of smart aptamers by methods of kinetic capillary electrophoresis |journal=Anal Chem. |volume=78 |issue=9 |pages=3171–8 |year=2006 |month=May |pmid=16643010 |doi=10.1021/ac060144h }} |
* {{cite journal |author=Drabovich AP, Berezovski M, Okhonin V, Krylov SN |title=Selection of smart aptamers by methods of kinetic capillary electrophoresis |journal=Anal Chem. |volume=78 |issue=9 |pages=3171–8 |year=2006 |month=May |pmid=16643010 |doi=10.1021/ac060144h }} |
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*{{cite journal |author=Cho EJ, Lee JW, Ellington, AD |title=Applications of Aptamers as Sensors |journal=Annual Review of Analytical Chemistry |volume=2 |issue=1 |pages=241–64 |year=2009 |pmid=20636061 |url=http://arjournals.annualreviews.org/doi/abs/10.1146/annurev.anchem.1.031207.112851 |doi=10.1146/annurev.anchem.1.031207.112851}} |
* {{cite journal |author=Cho EJ, Lee JW, Ellington, AD |title=Applications of Aptamers as Sensors |journal=Annual Review of Analytical Chemistry |volume=2 |issue=1 |pages=241–64 |year=2009 |pmid=20636061 |url=http://arjournals.annualreviews.org/doi/abs/10.1146/annurev.anchem.1.031207.112851 |doi=10.1146/annurev.anchem.1.031207.112851}} |
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{{refend}} |
{{refend}} |
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* [http://aptamer.freebase.com Aptamerska baza] |
* [http://aptamer.freebase.com Aptamerska baza] |
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* [http://immunet.cn/mimodb MimoDB baza podataka] |
* [http://immunet.cn/mimodb MimoDB baza podataka] |
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[[Kategorija:Nukleinske kiseline]] |
[[Kategorija:Nukleinske kiseline]] |
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Verzija na datum 22 juni 2014 u 15:18
Aptameri (od lat. aptus - stati, i grč. meros - region) su oligonukleinske kiseline ili peptidni molekuli koji se vezuju za specifični ciljni molekul. Prirodni aptameri postoje kao riboprekidači. U laboratoriji se aptameri opično formiraju selekcijom iz velikog mnoštva randomnih sekvenci. Aptameri nalaze primenu u baznim istraživanjima, a koriste se i za kliničke svrhe kao makromolekulski lekovi. Aptameri se mogu kombinovati sa ribozimima radi samostalnog presecanja u prisustvu ciljnog molekula.
Aptameri se mogu klasifikovati kao:
- DNK ili RNK ili KNK aptameri. Oni se obično sastoje od kratkih lanaca oligonukleotida.
- Peptidni aptameri. Oni se sastoje od kratkih promenljivih peptidnih domena, vezanih na oba kraja za proteinsku osnovu.
Literatura
- Ellington AD, Szostak JW (Aug 1990). „In vitro selection of RNA molecules that bind specific ligands”. Nature 346 (6287): 818–22. DOI:10.1038/346818a0. PMID 1697402.
- Bock LC, Griffin LC, Latham JA, Vermaas EH, Toole JJ (Feb 1992). „Selection of single-stranded DNA molecules that bind and inhibit human thrombin”. Nature 355 (6360): 564–6. DOI:10.1038/355564a0. PMID 1741036.
- Hoppe-Seyler F, Butz K (2000). „Peptide aptamers: powerful new tools for molecular medicine”. J Mol Med. 78 (8): 426–30. DOI:10.1007/s001090000140. PMID 11097111.
- Carothers JM, Oestreich SC, Davis JH, Szostak JW (Apr 2004). „Informational complexity and functional activity of RNA structures”. J Am Chem Soc. 126 (16): 5130–7. DOI:10.1021/ja031504a. PMID 15099096.
- Cohen BA, Colas P, Brent R (Nov 1998). „An artificial cell-cycle inhibitor isolated from a combinatorial library”. Proc Natl Acad Sci USA. 95 (24): 14272–7. DOI:10.1073/pnas.95.24.14272. PMC 24363. PMID 9826690.
- Binkowski BF, Miller RA, Belshaw PJ (Jul 2005). „Ligand-regulated peptides: a general approach for modulating protein-peptide interactions with small molecules”. Chem Biol. 12 (7): 847–55. DOI:10.1016/j.chembiol.2005.05.021. PMID 16039531.
- Sullenger BA, Gilboa E (Jul 2002). „Emerging clinical applications of RNA”. Nature 418 (6894): 252–8. DOI:10.1038/418252a. PMID 12110902.
- Ng EW, Shima DT, Calias P, Cunningham ET, Guyer DR, Adamis AP (Feb 2006). „Pegaptanib, a targeted anti-VEGF aptamer for ocular vascular disease”. Nat Rev Drug Discov 5 (2): 123–32. DOI:10.1038/nrd1955. PMID 16518379.
- Drabovich AP, Berezovski M, Okhonin V, Krylov SN (May 2006). „Selection of smart aptamers by methods of kinetic capillary electrophoresis”. Anal Chem. 78 (9): 3171–8. DOI:10.1021/ac060144h. PMID 16643010.
- Cho EJ, Lee JW, Ellington, AD (2009). „Applications of Aptamers as Sensors”. Annual Review of Analytical Chemistry 2 (1): 241–64. DOI:10.1146/annurev.anchem.1.031207.112851. PMID 20636061.