Premnaspirodien oksigenaza
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| Premnaspirodien oksigenaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.13.121 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Premnaspirodien oksigenaza (EC 1.14.13.121, HPO, Hyoscymus muticus premnaspirodienska oksigenaza) je enzim sa sistematskim imenom (-)-vetispiradien,NADPH:kiseonik 2alfa-oksidoreduktaza.[1] Ovaj enzim katalizuje sledeću hemijsku reakciju
- (-)-vetispiradien + 2 NADPH + 2 H+ + 2 O2 solavetivon + 2 NADP+ + 3H2O (sveukupna reakcija)
- (1a) (-)-vetispiradien + NADPH + H+ + O2 solavetivol + NADP+ +H2O
- (1b) solavetivol + NADPH + H+ + O2 solavetivon + NADP+ + 2H2O
Ovaj enzim je hem-tiolatni protein (P-450). Enzim iz biljke Hyoscymus muticus takođe hidroksiše valencen u C-2 poziciji dajući alfa-hidroksi jedinjenje, nootkatol, koje se dalje konvertuje u nootkaton.
Reference[uredi | uredi kod]
- ↑ Takahashi, S., Yeo, Y.S., Zhao, Y., O'Maille, P.E., Greenhagen, B.T., Noel, J.P., Coates, R.M. and Chappell, J. (2007). „Functional characterization of premnaspirodiene oxygenase, a cytochrome P450 catalyzing regio- and stereo-specific hydroxylations of diverse sesquiterpene substrates”. J. Biol. Chem. 282: 31744-31754. PMID 17715131.
Literatura[uredi | uredi kod]
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.