Piruvat oksidaza
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| Piruvat oksidaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.2.3.3 | ||||||||
| CAS broj | 9001-96-1 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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Piruvat oksidaza (EC 1.2.3.3, piruvinska oksidaza, fosfat-zavisna piruvatna oksidaza) je enzim sa sistematskim imenom piruvat:kiseonik 2-oksidoreduktaza (fosforilacija).[1][2] Ovaj enzim katalizuje sledeću hemijsku reakciju
Ovaj enzim je flavoprotein (FAD) za čiji rad je neophodan tiamin difosfat. Dva redukujuća ekvivalenta se prenose sa rezonantnih karbanjon/enaminskih formi 2-hidroksietil-tiamin-difosfata na susedni flavinski kofaktor, proizvodeći 2-acetil-tiamin difosfat (AcThDP) i redukovani flavin. FADH2 se reoksiduje posredstvom O2 čime se formira H2O2 i FAD. AcThDP se fosforolitički razlaže do acetil fosfata i tiamin difosfata.
Reference[uredi | uredi kod]
- ↑ Williams, F.R. and Hager, L.P. (1966). „Crystalline flavin pyruvate oxidase from Escherichia coli. I. Isolation and properties of the flavoprotein”. Arch. Biochem. Biophys. 116: 168-176. PMID 5336022.
- ↑ Tittmann, K., Wille, G., Golbik, R., Weidner, A., Ghisla, S. and Hübner, G. (2005). „Radical phosphate transfer mechanism for the thiamin diphosphate- and FAD-dependent pyruvate oxidase from Lactobacillus plantarum. Kinetic coupling of intercofactor electron transfer with phosphate transfer to acetyl-thiamin diphosphate via a transient FAD semiquinone/hydroxyethyl-ThDP radical pair”. Biochemistry 44: 13291-13303. PMID 16201755.
Literatura[uredi | uredi kod]
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.