Endopeptidaza-Clp
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| Endopeptidaza-Clp | |||||||||
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| Endopeptidaza-Clp homo14mer, Streptococcus pneumoniae | |||||||||
| Identifikatori | |||||||||
| EC broj | 3.4.21.92 | ||||||||
| CAS broj | 110910-59-3 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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Endopeptidaza-Clp (EC 3.4.21.92, endopeptidaza Ti, kazeinolitička proteaza, proteaza Ti, ATP-zavisna Clp proteaza, endopeptidaza Ti, kazeinolitička proteaza, ClpP, Clp proteaza) je enzim.[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
- Hidroliza proteina do malih peptida u presustvu ATP i Mg2+. Alfa-kazein je uobičajeni test supstrat. U odsustvu ATP, dolazi do hidrolize sam oligopeptida kraćih od pet ostataka (kao što je sukcinil-Leu-Tyr-NHMec; i Leu-Tyr-Leu-Tyr-Trp, u koje takođe dolazi do razlaganja -Tyr-Leu- i -Tyr-Trp veza)
Enzim iz bakterija sadrži dva tipa podjedinica, ClpP, sa peptidaznim dejstvom, i ClpA, sa ATPaznim dejstvom.
Reference[uredi | uredi kod]
- ↑ Gottesman, S., Clark, W.P. and Maurizi, M.R. (1990). „The ATP-dependent Clp protease of Escherichia coli. Sequence of clpA and identification of a Clp-specific substrate”. J. Biol. Chem. 265: 7886-7893. PMID 2186030.
- ↑ Maurizi, M.R., Clark, W.P., Katayama, Y., Rudikoff, S., Pumphrey, J., Bowers, B. and Gottesman, S. (1990). „Sequence and structure of Clp P, the proteolytic component of the ATP-dependent Clp protease of Escherichia coli”. J. Biol. Chem. 265: 12536-12545. PMID 2197275.
- ↑ Maurizi, M.R., Thompson, M.W., Singh, S.K. and Kim, S.-H. (1994). „Endopeptidase Clp: the ATP-dependent Clp protease from Escherichia coli”. Methods Enzymol. 244: 314-331. PMID 7845217.
- ↑ Kessel, M. (1995). „, Maurizi,M.R., Kim, B., Kocsis, E., Trus, B., Singh, S.K. and Steven, A.C. Homology in structural organization between E. coli ClpAP protease and the eukaryotic 26 S proteasome”. J. Mol. Biol. 250: 587-594. PMID 7623377.
Literatura[uredi | uredi kod]
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.