Amorpha-4,11-dienska sintaza

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Amorpha-4,11-dienska sintaza
Identifikatori
EC broj 4.2.3.24
CAS broj 259213-60-0
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures

Amorpha-4,11-dienska sintaza (EC 4.2.3.24, amorfadienska sintaza) je enzim sa sistematskim imenom (2E,6E)-farnezil-difosfat difosfat-lijaza (formira amorfa-4,11-dien).[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju

(2E,6E)-farnezil difosfat \rightleftharpoons amorfa-4,11-dien + difosfat

Za dejstvo ovog enzima su neophodni joni Mg2+ i Mn2+.

Reference[uredi - уреди]

  1. Wallaart, T.E., Bouwmeester, H.J., Hille, J., Poppinga, L. and Maijers, N.C. (2001). "Amorpha-4,11-diene synthase: cloning and functional expression of a key enzyme in the biosynthetic pathway of the novel antimalarial drug artemisinin". Planta 212: 460–465. PMID 11289612. 
  2. Mercke, P., Bengtsson, M., Bouwmeester, H.J., Posthumus, M.A. and Brodelius, P.E. (2000). "Molecular cloning, expression, and characterization of amorpha-4,11-diene synthase, a key enzyme of artemisinin biosynthesis in Artemisia annua L". Arch. Biochem. Biophys. 381: 173–180. PMID 11032404. 
  3. Bouwmeester, H.J., Wallaart, T.E., Janssen, M.H., van Loo, B., Jansen, B.J., Posthumus, M.A., Schmidt, C.O., De Kraker, J.W., König, W.A. and Franssen, M.C. (1999). "Amorpha-4,11-diene synthase catalyses the first probable step in artemisinin biosynthesis". Phytochemistry 52: 843–854. PMID 10626375. 
  4. Chang, Y.J., Song, S.H., Park, S.H. and Kim, S.U. (2000). "Amorpha-4,11-diene synthase of Artemisia annua: cDNA isolation and bacterial expression of a terpene synthase involved in artemisinin biosynthesis". Arch. Biochem. Biophys. 383: 178–184. PMID 11185551. 
  5. Martin, V.J., Pitera, D.J., Withers, S.T., Newman, J.D. and Keasling, J.D. (2003). "Engineering a mevalonate pathway in Escherichia coli for production of terpenoids". Nat. Biotechnol. 21: 796–802. PMID 12778056. 
  6. Picaud, S., Mercke, P., He, X., Sterner, O., Brodelius, M., Cane, D.E. and Brodelius, P.E. (2006). "Amorpha-4,11-diene synthase: Mechanism and stereochemistry of the enzymatic cyclization of farnesyl diphosphate". Arch. Biochem. Biophys. 448: 150–155. PMID 16143293. 

Literatura[uredi - уреди]

Vanjske veze[uredi - уреди]