Rizopuspepsin
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| Rizopuspepsin | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 3.4.23.21 | ||||||||
| CAS broj | 2620497 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Rizopuspepsin (EC 3.4.23.21, Rhizopus aspartinska proteinaza, neuraza, Rhizopus kiselinska proteaza, Rhizopus kiselinska proteinaza) je enzim.[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
- Hidroliza proteina sa širokom specifičnošću, slično pepsinu A. Postoji preferencija za hidrofobne ostatake u P1 i P1'. Dolazi do zgrušavanja mleka i aktivacije tripsinogena. Ne dolazi do razlaganja Gln4-His, ali se razlaže His10-Leu i Val12-Glu u B lancu insulina
Ovaj enzim je prisutan u gljivi Rhizopus chinensis.
Reference[uredi | uredi kod]
- ↑ Tsuru, D., Hattori, A., Tsuji, H., Yamamoto, T. and Fukumoto, J. (1969). „Studies on mold proteases. Part II. Substrate specificity of acid protease of Rhizopus chinensis”. Agric. Biol. Chem. 33: 1419-1426.
- ↑ Kurono, Y., Chidimatsu, M., Horikoshi, K. and Ikeda, Y. (1971). „Isolation of a protease from a Rhizopus product”. Agric. Biol. Chem. 35: 1668-1675.
- ↑ Ohtsuru, M., Tang, J. and Delaney, R. (1982). „Purification and characterization of rhizopuspesin isozymes from a liquid culture of Rhizopus chinensis”. Int. J. Biochem. 14: 925-932. PMID 6751894.
- ↑ Suguna, K., Padlan, E.A., Smith, C.W., Carlson, W.D. and Davies, D.R. (1987). „Binding of a reduced peptide inhibitor to the aspartic proteinase from Rhizopus chinensis: implications for a mechanism of action”. Proc. Natl. Acad. Sci. USA 84: 7009-7013. PMID 3313384.
Literatura[uredi | uredi kod]
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.