Ramnogalakturonanska hidrolaza
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| Ramnogalakturonanska hidrolaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 3.2.1.171 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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Ramnogalakturonanska hidrolaza (EC 3.2.1.171, ramnogalakturonaza A, RGaza A, RG-hidrolaza) je enzim sa sistematskim imenom ramnogalakturonan alfa-D-GalA-(1->2)-alfa-L-Rha hidrolaza.[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju
- Endohidroliza alfa-D-GalA-(1->2)-alfa-L-Rha glikozidne veze u ramnogalakturonan I osnovi sa inicijalnom inverzijom anomerne konfiguracije čime se odvajaju oligosaharidi sa beta-D-GalA ostatkom na redukujućem kraju
Ovaj enzim učestvuje u degradaciji ramnogalakturonana I kod Aspergillus aculeatus.
Reference[uredi | uredi kod]
- ↑ Petersen, T.N., Kauppinen, S. and Larsen, S. (1997). „The crystal structure of rhamnogalacturonase A from Aspergillus aculeatus: a right-handed parallel β helix”. Structure 5: 533-544. PMID 9115442.
- ↑ Kofod, L.V., Kauppinen, S., Christgau, S., Andersen, L.N., Heldt-Hansen, H.P., Dorreich, K. and Dalboge, H. (1994). „Cloning and characterization of two structurally and functionally divergent rhamnogalacturonases from Aspergillus aculeatus”. J. Biol. Chem. 269: 29182-29189. PMID 7961884.
- ↑ Azadi, P., O'Neill, M.A., Bergmann, C., Darvill, A.G. and Albersheim, P. (1995). „The backbone of the pectic polysaccharide rhamnogalacturonan I is cleaved by an endohydrolase and an endolyase”. Glycobiology 5: 783-789. PMID 8720076.
- ↑ Petersen, T.N., Christgau, S., Kofod, L.V., Kauppinen, S., Johnson, A.H. and Larsen, S. (1997). „Crystallization and preliminary X-ray studies of rhamnogalacturonase A from Aspergillus aculeatus”. Acta Crystallogr. D Biol. Crystallogr. 53: 105-107. PMID 15299976.
- ↑ Pitson, S.M., Mutter, M., van den Broek, L.A., Voragen, A.G. and Beldman, G. (1998). „Stereochemical course of hydrolysis catalysed by α-L-rhamnosyl and α-D-galacturonosyl hydrolases from Aspergillus aculeatus”. Biochem. Biophys. Res. Commun. 242: 552-559. PMID 9464254.
Literatura[uredi | uredi kod]
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.