Pseudomonalizin

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Pseudomonalizin
Identifikatori
EC broj 3.4.21.100
CAS broj 848318-58-1
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures

Pseudomonalizin (EC 3.4.21.100, Pseudomonas sp. pepstatin-insenzitivna karboksilna proteinaza, pseudomonapepsin, sedolizin) je enzim.[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju

Hidroliza B lanca insulina na -Glu13-Ala-, -Leu15-Tyr- i -Phe25-Tyr-, i angiotenzina I na -Tyr4-Ile-. Dobar sintetički supstrat je Lys-Pro-Ile-Glu-Phe-Phe(NO2)-Arg-Leu.

Ovaj enzim izlučuje Pseudomonas sp. No. 101. Optimalni pH je 4.

Reference[uredi - уреди | uredi izvor]

  1. Oda, K., Sugitani, M., Fukuhara, K. and Murao, S. (1987). "Purification and properties of a pepstatin-insensitive carboxyl proteinase from a Gram-negative bacterium". Biochim. Biophys. Acta 923: 463–469. PMID 3548827. 
  2. Oda, K., Nakatani, H. and Dunn, B.M. (1992). "Substrate specificity and kinetic properties of pepstatin-insensitive carboxyl proteinase from Pseudomonas sp. No. 101". Biochim. Biophys. Acta 1120: 208–214. PMID 1562589. 
  3. Wlodawer, A., Li, M., Dauter, Z., Gustchina, A., Uchida, K., Oyama, H., Dunn, B.M. and Oda, K. (2001). "Carboxyl proteinase from Pseudomonas defines a novel family of subtilisin-like enzymes". Nat. Struct. Biol. 8: 442–446. PMID 11323721. 
  4. Wlodawer, A., Li, M., Gustchina, A., Oyama, H., Dunn, B.M. and Oda, K. (2003). "Structural and enzymatic properties of the sedolisin family of serine-carboxyl peptidases". Acta Biochim. Pol. 50: 81–102. PMID 12673349. 

Literatura[uredi - уреди | uredi izvor]

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