Piridoksal 5'-fosfatna sintaza (hidroliza glutamina)
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| Piridoksal 5'-fosfatna sintaza (hidroliza glutamina) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 4.3.3.6 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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Piridoksal 5'-fosfatna sintaza (hidroliza glutamina) (EC 4.3.3.6, PdxST) je enzim sa sistematskim imenom D-riboza 5-fosfat,D-gliceraldehid 3-fosfat piridoksal 5'-fosfat-lijaza.[1][2][3][4][5][6][7][8] Ovaj enzim katalizuje sledeću hemijsku reakciju
- D-riboza 5-fosfat + D-gliceraldehid 3-fosfat + L-glutamin piridoksal 5'-fosfat + L-glutamat + 3H2O + fosfat (sveukupna reakcija)
- (1a) L-glutamin + H2O L-glutamat + NH3
- (1b) D-riboza 5-fosfat + D-gliceraldehid 3-fosfat + NH3 piridoksal 5'-fosfat + 4H2O + fosfat
Amonijak obezbeđuje glutaminazna podjedinica.
Reference[uredi | uredi kod]
- ↑ Burns, K.E., Xiang, Y., Kinsland, C.L., McLafferty, F.W. and Begley, T.P. (2005). „Reconstitution and biochemical characterization of a new pyridoxal-5′-phosphate biosynthetic pathway”. J. Am. Chem. Soc. 127: 3682-3683. PMID 15771487.
- ↑ Raschle, T., Amrhein, N. and Fitzpatrick, T.B. (2005). „On the two components of pyridoxal 5′-phosphate synthase from Bacillus subtilis”. J. Biol. Chem. 280: 32291-32300. PMID 16030023.
- ↑ Strohmeier, M., Raschle, T., Mazurkiewicz, J., Rippe, K., Sinning, I., Fitzpatrick, T.B. and Tews, I. (2006). „Structure of a bacterial pyridoxal 5′-phosphate synthase complex”. Proc. Natl. Acad. Sci. USA 103: 19284-19289. PMID 17159152.
- ↑ Raschle, T., Arigoni, D., Brunisholz, R., Rechsteiner, H., Amrhein, N. and Fitzpatrick, T.B. (2007). „Reaction mechanism of pyridoxal 5′-phosphate synthase. Detection of an enzyme-bound chromophoric intermediate”. J. Biol. Chem. 282: 6098-6105. PMID 17189272.
- ↑ Hanes, J.W., Keresztes, I. and Begley, T.P. (2008). „Trapping of a chromophoric intermediate in the Pdx1-catalyzed biosynthesis of pyridoxal 5′-phosphate”. Angew. Chem. Int. Ed. Engl. 47: 2102-2105. PMID 18260082.
- ↑ Hanes, J.W., Burns, K.E., Hilmey, D.G., Chatterjee, A., Dorrestein, P.C. and Begley, T.P. (2008). „Mechanistic studies on pyridoxal phosphate synthase: the reaction pathway leading to a chromophoric intermediate”. J. Am. Chem. Soc. 130: 3043-3052. PMID 18271580.
- ↑ Hanes, J.W., Keresztes, I. and Begley, T.P. (2008). „13C NMR snapshots of the complex reaction coordinate of pyridoxal phosphate synthase”. Nat. Chem. Biol. 4: 425-430. PMID 18516049.
- ↑ Wallner, S., Neuwirth, M., Flicker, K., Tews, I. and Macheroux, P. (2009). „Dissection of contributions from invariant amino acids to complex formation and catalysis in the heteromeric pyridoxal 5-phosphate synthase complex from Bacillus subtilis”. Biochemistry 48: 1928-1935. PMID 19152323.
Literatura[uredi | uredi kod]
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.