Pestivirusna NS3 poliproteinska peptidaza
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| Pestivirusna NS3 poliproteinska peptidaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 3.4.21.113 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Pestivirusna NS3 poliproteinska peptidaza (EC 3.4.21.113, endopeptidaza virusa NS3 granične bolesti, BDV NS3 endopeptidaza, endopeptidaza virusa NS3 goveđe virusne dijareje, BVDV NS3 endopeptidaza, endopeptidaza virusa NS3 klasične svinjske groznice, CSFV NS3 endopeptidaza, p80) je enzim.[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
- Leu je konzerviran u poziciji P1 za sva četiri mesta presecanja. Alanin je nađen u poziciji P1' mesta razlaganja: NS4A-NS4B, dok je serin u poziciji P1' u mestima NS3-NS4A, NS4B-NS5A i NS5A-NS5B
Poliprotein necitopatogenug pestivirusa se preseca ko- i posttranslaciono u najmanje 11 proteina (Npro, C, Erns, E1, E2, p7, NS2-3, NS4A, NS4B, NS5A, i NS5B).
Reference[uredi | uredi kod]
- ↑ Wiskerchen, M. and Collett, M.S. (1991). „Pestivirus gene expression: protein p80 of bovine viral diarrhea virus is a proteinase involved in polyprotein processing”. Virology 184: 341-350. PMID 1651596.
- ↑ Tautz, N., Elbers, K., Stoll, D., Meyers, G. and Thiel, H.J. (1997). „Serine protease of pestiviruses: determination of cleavage sites”. J. Virol. 71: 5415-5422. PMID 9188613.
- ↑ Xu, J., Mendez, E., Caron, P.R., Lin, C., Murcko, M.A., Collett, M.S. and Rice, C.M. (1997). „Bovine viral diarrhea virus NS3 serine proteinase: polyprotein cleavage sites, cofactor requirements, and molecular model of an enzyme essential for pestivirus replication”. J. Virol. 71: 5312-5322. PMID 9188600.
- ↑ Tautz, N., Kaiser, A. and Thiel, H.J. (2000). „NS3 serine protease of bovine viral diarrhea virus: characterization of active site residues, NS4A cofactor domain, and protease-cofactor interactions”. Virology 273: 351-363. PMID 10915606.
Literatura[uredi | uredi kod]
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.