Pektinska lijaza
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| Pektinska lijaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 4.2.2.10 | ||||||||
| CAS broj | 9033-35-6 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Pektinska lijaza (EC 4.2.2.10, pectin trans-eliminaza, endo-pektin lijaza, polimetilgalacturonska transeliminaza, pectin metiltranseliminaza, pectolijaza, PL, PNL, PMGL) je enzim sa sistematskim imenom (1->4)-6-O-metil-alfa-D-galakturonan lijaza.[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
- Eliminativno razlaganje (1->4)-alfa-D-galakturonan metil estra čime se formiraju oligosaharidi sa 4-dezoksi-6-O-metil-alfa-D-galakt-4-enuronozil grupama na njihovim neredukujućim krajevima
Ovaj enzim je reaktivniji na pektinu u odnosu na pectat.
Reference[uredi | uredi kod]
- ↑ Albersheim, P., Neukom, H. and Deuel, H. (1960). „Über die Bildung von ungesättigten Abbauprodukten durch ein pekinabbauendes Enzym”. Helv. Chim. Acta 43: 1422-1426.
- ↑ Mayans, O., Scott, M., Connerton, I., Gravesen, T., Benen, J., Visser, J., Pickersgill, R. and Jenkins, J. (1997). „Two crystal structures of pectin lyase A from Aspergillus reveal a pH driven conformational change and striking divergence in the substrate-binding clefts of pectin and pectate lyases”. Structure 5: 677-689. PMID 9195887.
- ↑ Kester, H.C.M and Visser, J. (1994). „Purification and characterization of pectin lyase B, a novel pectinolytic enzyme from Aspergillus niger”. FEMS Microbiol. Lett. 120: 63-68.
- ↑ Mutenda, K.E., Körner, R., Christensen, T.M.I.E., Mikkelsen, J. and Roepstorff, P. (2002). „Application of mass spectrometry to determine the activity and specificity of pectin lyase A”. Carbohydr. Res. 337: 1213-1223. PMID 12110197.
Literatura[uredi | uredi kod]
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.