Nespecifična poliamin oksidaza
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| Nespecifična poliamin oksidaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.5.3.17 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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Nespecifična poliamin oksidaza (EC 1.5.3.17, poliaminska oksidaza (nespecifična), Fms1, AtPAO3) je enzim sa sistematskim imenom poliamin:kiseonik oksidoreduktaza (formira 3-aminopropanal ili 3-acetamidopropanal).[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
- (1) spermin + O2 +H2O spermidin + 3-aminopropanal +H2O2
- (2) spermidin + O2 +H2O putrescin + 3-aminopropanal +H2O2
- (3) N1-acetilspermin + O2 +H2O spermidin + 3-acetamidopropanal +H2O2
- (4) N1-acetilspermidin + O2 +H2O putrescin + 3-acetamidopropanal +H2O2
Ovaj enzim je flavoprotein (FAD). Ova nespecifična poliaminska oksidaza se može znatno razlikovati od drugih enzima ovog tipa. Enzim iz Saccharomyces cerevisiae ima veoma široku specifičnost. On takođe oksiduje N8-acetilspermidin. Enzim iz Ascaris suum je veoma aktivan na sperminu i spermidinu. On takođe oksiduje norspermin.
Reference[uredi | uredi kod]
- ↑ Moschou, P.N., Sanmartin, M., Andriopoulou, A.H., Rojo, E., Sanchez-Serrano, J.J. and Roubelakis-Angelakis, K.A. (2008). „Bridging the gap between plant and mammalian polyamine catabolism: a novel peroxisomal polyamine oxidase responsible for a full back-conversion pathway in Arabidopsis”. Plant Physiol. 147: 1845-1857. PMID 18583528.
- ↑ Muller, S. and Walter, R.D. (1992). „Purification and characterization of polyamine oxidase from Ascaris suum”. Biochem. J. 283: 75-80. PMID 1567380.
- ↑ Landry, J. and Sternglanz, R. (2003). „Yeast Fms1 is a FAD-utilizing polyamine oxidase”. Biochem. Biophys. Res. Commun. 303: 771-776. PMID 12670477.
Literatura[uredi | uredi kod]
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.