Hijaluronan sintaza
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| Hijaluronan sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.4.1.212 | ||||||||
| CAS broj | 39346-43-5 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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Hijaluronan sintaza (EC 2.4.1.212, spHAS, seHAS) je enzim sa sistematskim imenom Naizmenični UDP-alfa-N-acetil-D-glukozamin:beta-D-glukuronazil-(1->3)-(nascent hijaluronan) 4-N-acetil-beta-D-glukozaminiltransferaza i UDP-alfa-D-glukuronat:N-acetil-beta-D-glukozaminil-(1->4)-(nascentni hijaluronan) 3-beta-D-glukuronaziltransferaza.[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
- (1) UDP-alfa-N-acetil-D-glukozamin + beta-D-glukuronazil-(1->3)-N-acetil-beta-D-glukozaminil-(1->4)-[nascentni hijaluronan] UDP + N-acetil-beta-D-glukozaminil-(1->4)-beta-D-glukuronazil-(1->3)-N-acetil-beta-D-glukozaminil-(1->4)-[nascentni hijaluronan]
- (2) UDP-alfa-D-glukuronat + N-acetil-beta-D-glukozaminil-(1->4)-beta-D-glukuronazil-(1->3)-[nascentni hijaluronan] UDP + beta-D-glukuronazil-(1->3)-N-acetil-beta-D-glukozaminil-(1->4)-beta-D-glukuronazil-(1->3)-[nascentni hijaluronan]
Za dejstvo enzima iz Streptococcus grupe A i grupe C je neophodan Mg2+.
Reference[uredi | uredi kod]
- ↑ DeAngelis, P.L., Papaconstantinou, J. and Weigel, P.H. (1993). „Molecular cloning, identification and sequence of the hyaluronan synthase gene from Group A Streptococcus pyogenes”. J. Biol. Chem. 268: 19181-19184. PMID 8366070.
- ↑ Jing, W. and DeAngelis, P.L. (2000). „Dissection of the two transferase activities of the Pasteurella multocida hyaluronan synthase: two active sites exist in one polypeptide”. Glycobiology 10: 883-889. PMID 10988250.
- ↑ DeAngelis, P.L. (1999). „Molecular directionality of polysaccharide polymerization by the Pasteurella multocida hyaluronan synthase”. J. Biol. Chem. 274: 26557-26562. PMID 10473619.
- ↑ Tlapak-Simmons, V.L., Baron, C.A. and Weigel, P.H. (2004). „Characterization of the purified hyaluronan synthase from Streptococcus equisimilis”. Biochemistry 43: 9234-9242. PMID 15248781.
Literatura[uredi | uredi kod]
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.