Glutamin-(asparagin-)aza

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Glutamin-(asparagin-)aza
Identifikatori
EC broj 3.5.1.38
CAS broj 39335-03-0
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures

Glutamin-(asparagin-)aza (EC 3.5.1.38, glutaminaza-asparaginaza, ansB (gen), L-asparagin/L-glutamin amidohidrolaza, L-ASNaza/L-GLNaza) je enzim sa sistematskim imenom L-glutamin(L-asparagin) amidohidrolaza.[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju

(1) L-glutamin + H2O L-glutamat + NH3
(2) L-asparagin + H2O L-aspartat + NH3

Enzim iz bakterije Achromobacter hidrolizuje L-asparagin sa 0.8 brzine hidrolize L-glutamina.

Reference[uredi - уреди | uredi izvor]

  1. Roberts, J., Holcenberg, J.S. and Dolowy, W.C. (1972). "Isolation, crystallization, and properties of Achromobacteraceae glutaminase-asparaginase with antitumor activity". J. Biol. Chem. 247: 84–90. PMID 5017769. 
  2. Tanaka, S., Robinson, E.A., Appella, E., Miller, M., Ammon, H.L., Roberts, J., Weber, I.T. and Wlodawer, A. (1988). "Structures of amidohydrolases. Amino acid sequence of a glutaminase-asparaginase from Acinetobacter glutaminasificans and preliminary crystallographic data for an asparaginase from Erwinia chrysanthemi". J. Biol. Chem. 263: 8583–8591. PMID 3379033. 
  3. Lubkowski, J., Wlodawer, A., Ammon, H.L., Copeland, T.D. and Swain, A.L. (1994). "Structural characterization of Pseudomonas 7A glutaminase-asparaginase". Biochemistry 33: 10257–10265. PMID 8068664. 
  4. Ortlund, E., Lacount, M.W., Lewinski, K. and Lebioda, L. (2000). "Reactions of Pseudomonas 7A glutaminase-asparaginase with diazo analogues of glutamine and asparagine result in unexpected covalent inhibitions and suggests an unusual catalytic triad Thr-Tyr-Glu". Biochemistry 39: 1199–1204. PMID 10684596. 

Literatura[uredi - уреди | uredi izvor]

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