Ent-kauren oksidaza
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| Ent-kauren oksidaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.13.78 | ||||||||
| CAS broj | 149565-67-3 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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Ent-kauren oksidaza (EC 1.14.13.78, ent-kaurenska oksidaza) je enzim sa sistematskim imenom ent-kaur-16-en,NADPH:kiseonik oksidoreduktaza (hidroksilacija).[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
- ent-kaur-16-en + 3 NADPH + 3 H+ + 3 O2 ent-kaur-16-en-19-oat + 3 NADP+ + 4H2O (sveukupna reakcija)
- (1a) ent-kaur-16-en + NADPH + H+ + O2 ent-kaur-16-en-19-ol + NADP+ + H2O
- (1b) ent-kaur-16-en-19-ol + NADPH + H+ + O2 ent-kaur-16-en-19-al + NADP+ + 2H2O
- (1c) ent-kaur-16-en-19-al + NADPH + O2 ent-kaur-16-en-19-oat + NADP+ + H2O
Za rad ovog enzima je neophodan citohrom P450. On katalizuje tri sukcesivne oksidacije 4-metil grupe ent-kaurena dajući kaurenoinsku kiselinu.
Reference[uredi | uredi kod]
- ↑ Ashman, P.J., Mackenzie, A. and Bramley, P.M. (1990). „Characterization of ent-kaurene oxidase activity from Gibberella fujikuroi”. Biochim. Biophys. Acta 1036: 151-157. PMID 2223832.
- ↑ Archer, C., Ashman, P.J., Hedden, P., Bowyer, J.R. and Bramley, P.M. (1992). „Purification of ent-kaurene oxidase from Gibberella fujikuroi and Cucurbita maxima”. Biochem. Soc. Trans. 20: 218-218. PMID 1397591.
- ↑ Helliwell, C.A., Poole, A., Peacock, W.J. and Dennis, E.S. (1999). „Arabidopsis ent-kaurene oxidase catalyzes three steps of gibberellin biosynthesis”. Plant Physiol. 119: 507-510. PMID 9952446.
Literatura[uredi | uredi kod]
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.