Pseudomonalizin
(Preusmjereno sa stranice EC 3.4.21.100)
| Pseudomonalizin | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 3.4.21.100 | ||||||||
| CAS broj | 848318-58-1 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Pseudomonalizin (EC 3.4.21.100, Pseudomonas sp. pepstatin-insenzitivna karboksilna proteinaza, pseudomonapepsin, sedolizin) je enzim.[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
- Hidroliza B lanca insulina na -Glu13-Ala-, -Leu15-Tyr- i -Phe25-Tyr-, i angiotenzina I na -Tyr4-Ile-. Dobar sintetički supstrat je Lys-Pro-Ile-Glu-Phe-Phe(NO2)-Arg-Leu.
Ovaj enzim izlučuje Pseudomonas sp. No. 101. Optimalni pH je 4.
Reference[uredi | uredi kod]
- ↑ Oda, K., Sugitani, M., Fukuhara, K. and Murao, S. (1987). „Purification and properties of a pepstatin-insensitive carboxyl proteinase from a Gram-negative bacterium”. Biochim. Biophys. Acta 923: 463-469. PMID 3548827.
- ↑ Oda, K., Nakatani, H. and Dunn, B.M. (1992). „Substrate specificity and kinetic properties of pepstatin-insensitive carboxyl proteinase from Pseudomonas sp. No. 101”. Biochim. Biophys. Acta 1120: 208-214. PMID 1562589.
- ↑ Wlodawer, A., Li, M., Dauter, Z., Gustchina, A., Uchida, K., Oyama, H., Dunn, B.M. and Oda, K. (2001). „Carboxyl proteinase from Pseudomonas defines a novel family of subtilisin-like enzymes”. Nat. Struct. Biol. 8: 442-446. PMID 11323721.
- ↑ Wlodawer, A., Li, M., Gustchina, A., Oyama, H., Dunn, B.M. and Oda, K. (2003). „Structural and enzymatic properties of the sedolisin family of serine-carboxyl peptidases”. Acta Biochim. Pol. 50: 81-102. PMID 12673349.
Literatura[uredi | uredi kod]
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.