DNK polimeraza beta

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DNK polimeraza beta

PDB prikaz baziran na 1bno.
Dostupne strukture
1BPX, 1BPY, 1BPZ, 1MQ2, 1MQ3, 1TV9, 1TVA, 1ZJM, 1ZJN, 1ZQA, 1ZQB, 1ZQC, 1ZQD, 1ZQE, 1ZQF, 1ZQG, 1ZQH, 1ZQI, 1ZQJ, 1ZQK, 1ZQL, 1ZQM, 1ZQN, 1ZQO, 1ZQP, 1ZQQ, 1ZQR, 1ZQS, 1ZQT, 2FMP, 2FMQ, 2FMS, 2I9G, 2ISO, 2ISP, 2P66, 2PXI, 3C2K, 3C2L, 3C2M, 3GDX, 3ISB, 3ISC, 3ISD, 3JPN, 3JPO, 3JPP, 3JPQ, 3JPR, 3JPS, 3JPT, 3LK9, 3MBY, 3OGU, 3RH4, 3RH5, 3RH6, 3RJE, 3RJF, 3RJG, 3RJH, 3RJI, 3RJJ, 3RJK, 3TFR, 3TFS, 7ICE, 7ICF, 7ICG, 7ICH, 7ICI, 7ICJ, 7ICK, 7ICL, 7ICM, 7ICN, 7ICO, 7ICP, 7ICQ, 7ICR, 7ICS, 7ICT, 7ICU, 7ICV, 8ICA, 8ICB, 8ICC, 8ICE, 8ICF, 8ICG, 8ICH, 8ICI, 8ICJ, 8ICK, 8ICL, 8ICM, 8ICN, 8ICO, 8ICP, 8ICQ, 8ICR, 8ICS, 8ICT, 8ICU, 8ICV, 8ICW, 8ICX, 8ICY, 8ICZ, 9ICA, 9ICB, 9ICC, 9ICE, 9ICF, 9ICG, 9ICH, 9ICI, 9ICJ, 9ICK, 9ICL, 9ICM, 9ICN, 9ICO, 9ICP, 9ICQ, 9ICR, 9ICS, 9ICT, 9ICU, 9ICV, 9ICW, 9ICX, 9ICY
Identifikatori
SimboliPOLB; MGC125976
Vanjski IDOMIM174760 MGI97740 HomoloGene2013 GeneCards: POLB Gene
EC broj2.7.7.7
Pregled RNK izražavanja
PBB GE POLB 203616 at tn.png
podaci
Ortolozi
VrstaČovekMiš
Entrez542318970
EnsemblENSG00000070501ENSMUSG00000031536
UniProtP06746Q8K409
RefSeq (mRNA)NM_002690.2NM_011130.2
RefSeq (protein)NP_002681.1NP_035260.1
Lokacija (UCSC)Chr 8:
42.2 - 42.23 Mb
Chr 8:
23.74 - 23.76 Mb
PubMed pretraga[1][2]
Regulatorni element u POLB
RF01455.png
Predviđena sekundarna struktura stem loopII (M2) regulatornog elementa POLB
Identifikatori
Simbol POLB
Rfam RF01455
Entrez 5423
HUGO POLB
OMIM 174760
RefSeq NM_002690
Drugi podaci
RNK tip Cis-reg
Domain(i) Mammalia
Lokus Hromozom 8 p11.2

DNK polimeraza beta (POLB) je enzim koji kod ljudi kodira POLB gen.[1]

Funkcija[uredi - уреди | uredi izvor]

U eukariotskim ćelijama, DNK polimeraza beta (POLB) izvodi popravke ekscizijom baza (BER) koje su neophodne za popravku DNK, replikaciju, rekombinaciju, i otpornost na lekove.[1]

Regulacija ekspresije[uredi - уреди | uredi izvor]

DNK polimeraza beta održava genomski integritet putem uzimanja učešća u popravci ekscizijom baza. Prekomerno izražavanje POLB iRNK je u korelaciji sa brojnim tipovima kancera, dok deficijencije POLB-a rezultuju u hipersenzitivnosti na alkilirajuće agense, uključujući apoptozu, i prekid hromozoma.[2] Iz tih razloga, esencijalno je precizno održavanje kontrole POLB ekspresije.[3][4][5][6]

Interakcije[uredi - уреди | uredi izvor]

DNA polimeraza beta formira interakcije sa PNKP[7] i XRCC1.[8][9][10][11]

Reference[uredi - уреди | uredi izvor]

  1. 1,0 1,1 "Entrez Gene: POLB polymerase (DNA directed), beta". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=5423. 
  2. Narayan S, He F, Wilson SH (August 1996). "Activation of the human DNA polymerase beta promoter by a DNA-alkylating agent through induced phosphorylation of cAMP response element-binding protein-1". J. Biol. Chem. 271 (31): 18508–13. PMID 8702497. doi:10.1074/jbc.271.31.18508. 
  3. Canitrot Y; Cazaux C; Fréchet M et al. (October 1998). "Overexpression of DNA polymerase beta in cell results in a mutator phenotype and a decreased sensitivity to anticancer drugs". Proc. Natl. Acad. Sci. U.S.A. 95 (21): 12586–90. PMC 22874. PMID 9770529. doi:10.1073/pnas.95.21.12586. 
  4. Bergoglio V; Pillaire MJ; Lacroix-Triki M et al. (June 2002). "Deregulated DNA polymerase beta induces chromosome instability and tumorigenesis". Cancer Res. 62 (12): 3511–4. PMID 12067997. 
  5. Bergoglio V; Canitrot Y; Hogarth L et al. (September 2001). "Enhanced expression and activity of DNA polymerase beta in human ovarian tumor cells: impact on sensitivity towards antitumor agents". Oncogene 20 (43): 6181–7. PMID 11593426. doi:10.1038/sj.onc.1204743. 
  6. Srivastava DK, Husain I, Arteaga CL, Wilson SH (June 1999). "DNA polymerase beta expression differences in selected human tumors and cell lines". Carcinogenesis 20 (6): 1049–54. PMID 10357787. doi:10.1093/carcin/20.6.1049. 
  7. Whitehouse, C J; Taylor R M, Thistlethwaite A, Zhang H, Karimi-Busheri F, Lasko D D, Weinfeld M, Caldecott K W (January 2001). "XRCC1 stimulates human polynucleotide kinase activity at damaged DNA termini and accelerates DNA single-strand break repair". Cell (United States) 104 (1): 107–17. ISSN 0092-8674. PMID 11163244. doi:10.1016/S0092-8674(01)00195-7. 
  8. Wang, Liming; Bhattacharyya Nandan, Chelsea Diane M, Escobar Pedro F, Banerjee Sipra (November 2004). "A novel nuclear protein, MGC5306 interacts with DNA polymerase beta and has a potential role in cellular phenotype". Cancer Res. (United States) 64 (21): 7673–7. ISSN 0008-5472. PMID 15520167. doi:10.1158/0008-5472.CAN-04-2801. 
  9. Fan, Jinshui; Otterlei Marit, Wong Heng-Kuan, Tomkinson Alan E, Wilson David M (2004). "XRCC1 co-localizes and physically interacts with PCNA". Nucleic Acids Res. (England) 32 (7): 2193–201. PMC 407833. PMID 15107487. doi:10.1093/nar/gkh556. 
  10. Kubota, Y; Nash R A, Klungland A, Schär P, Barnes D E, Lindahl T (December 1996). "Reconstitution of DNA base excision-repair with purified human proteins: interaction between DNA polymerase beta and the XRCC1 protein". EMBO J. (ENGLAND) 15 (23): 6662–70. ISSN 0261-4189. PMC 452490. PMID 8978692. 
  11. Bhattacharyya, N; Banerjee S (July 2001). "A novel role of XRCC1 in the functions of a DNA polymerase beta variant". Biochemistry (United States) 40 (30): 9005–13. ISSN 0006-2960. PMID 11467963. doi:10.1021/bi0028789. 

Literatura[uredi - уреди | uredi izvor]

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