Adenozilkobinamid-fosfat guanililtransferaza
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| Adenozilkobinamid-fosfat guanililtransferaza | |||||||||
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| Identifikatori | |||||||||
| EC broj | 2.7.7.62 | ||||||||
| CAS broj | 169592-55-6 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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Adenozilkobinamid-fosfat guanililtransferaza (EC 2.7.7.62, CobU, adenozilkobinamid kinaza/adenozilkobinamid-fosfat guanililtransferaza, AdoCbi kinaza/AdoCbi-fosfat guanililtransferaza) je enzim sa sistematskim imenom GTP:adenozilkobinamid-fosfat guanililtransferaza.[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju
- GTP + adenozilkobinamid fosfat difosfat + adenozilkobinamid-GDP
Kod Salmonella typhimurium LT2 pod anaerobnim uslovima, CobU (EC 2.7.7.62 i EC 2.7.1.156), CobT (EC 2.4.2.21), CobC (EC 3.1.3.73) i CobS (EC 2.7.8.26) katalizuju reakcije kojima se formira nukleotidna petlja, čime se konvertuje adenozilkobinamid (AdoCbi) u adenozilkobalamin (AdoCbl).
Reference[uredi | uredi kod]
- ↑ O'Toole, G.A. and Escalante-Semerena, J.C. (1995). „Purification and characterization of the bifunctional CobU enzyme of Salmonella typhimurium LT2. Evidence for a CobU-GMP intermediate”. J. Biol. Chem. 270: 23560-23569. PMID 7559521.
- ↑ Thompson, T.B., Thomas, M.G., Escalante-Semerena, J.C. and Rayment, I. (1998). „Three-dimensional structure of adenosylcobinamide kinase/adenosylcobinamide phosphate guanylyltransferase from Salmonella typhimurium determined to 2.3 Å resolution”. Biochemistry 37: 7686-7695. PMID 9601028.
- ↑ Thompson, T.B., Thomas, M.G., Escalante-Semerena, J.C. and Rayment, I. (1999). „Three-dimensional structure of adenosylcobinamide kinase/adenosylcobinamide phosphate guanylyltransferase (CobU) complexed with GMP: evidence for a substrate-induced transferase active site”. Biochemistry 38: 12995-13005. PMID 10529169.
- ↑ Thomas, M.G., Thompson, T.B., Rayment, I. and Escalante-Semerena, J.C. (2000). „Analysis of the adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase (CobU) enzyme of Salmonella typhimurium LT2. Identification of residue His-46 as the site of guanylylation”. J. Biol. Chem. 275: 27576-27586. PMID 10869342.
- ↑ Warren, M.J., Raux, E., Schubert, H.L. and Escalante-Semerena, J.C. (2002). „The biosynthesis of adenosylcobalamin (vitamin B12)”. Nat. Prod. Rep. 19: 390-412. PMID 12195810.
Literatura[uredi | uredi kod]
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.