5-Beta-holestan-3-alfa,7-alfa-diol 12-alfa-hidroksilaza
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| 5-Beta-holestan-3-alfa,7-alfa-diol 12-alfa-hidroksilaza | |||||||||
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| Identifikatori | |||||||||
| EC broj | 1.14.13.96 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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5-Beta-holestan-3-alfa,7-alfa-diol 12-alfa-hidroksilaza (EC 1.14.13.96, 5beta-holestan-3alfa,7alfa-diol 12alfa-monooksigenaza, sterol 12alfa-hidroksilaza (nespecifična), CYP8B1, citohrom P450 8B1) je enzim sa sistematskim imenom 5beta-holestan-3alfa,7alfa-diol,NADPH:kiseonik oksidoreduktaza (12alfa-hidroksilacija).[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju
- 5beta-holestan-3alfa,7alfa-diol + NADPH + H+ + O2 5beta-holestan-3alfa,7alfa,12alfa-triol + NADP+ +H2O
Ovaj enzim je hem-tiolatni protein (P-450). On je ključni enzim u biosintezi žučne holinske kiselina (3alfa,7alfa,12alfa-trihidroksi-5beta-holanoinska kiselina).
Reference[uredi | uredi kod]
- ↑ Hansson, R. and Wikvall, K. (1982). „Hydroxylations in biosynthesis of bile acids. Cytochrome P-450 LM4 and 12α-hydroxylation of 5β-cholestane-3α,7α-diol”. Eur. J. Biochem. 125: 423-429. PMID 6811268.
- ↑ Hansson, R. and Wikvall, K. (1980). „Hydroxylations in biosynthesis and metabolism of bile acids. Catalytic properties of different forms of cytochrome P-450”. J. Biol. Chem. 255: 1643-1649. PMID 6766451.
- ↑ Lundell, K. and Wikvall, K. (2003). „Gene structure of pig sterol 12α-hydroxylase (CYP8B1) and expression in fetal liver: comparison with expression of taurochenodeoxycholic acid 6α-hydroxylase (CYP4A21)”. Biochim. Biophys. Acta 1634: 86-96. PMID 14643796.
- ↑ del Castillo-Olivares, A. and Gil, G. (2000). „α1-Fetoprotein transcription factor is required for the expression of sterol 12α -hydroxylase, the specific enzyme for cholic acid synthesis. Potential role in the bile acid-mediated regulation of gene transcription”. J. Biol. Chem. 275: 17793-17799. PMID 10747975.
- ↑ Yang, Y., Zhang, M., Eggertsen, G. and Chiang, J.Y. (2002). „On the mechanism of bile acid inhibition of rat sterol 12α-hydroxylase gene (CYP8B1) transcription: roles of α-fetoprotein transcription factor and hepatocyte nuclear factor 4alpha”. Biochim. Biophys. Acta 1583: 63-73. PMID 12069850.
- ↑ Russell, D.W. (2003). „The enzymes, regulation, and genetics of bile acid synthesis”. Annu. Rev. Biochem. 72: 137-174. PMID 12543708.
Literatura[uredi | uredi kod]
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.