3,4-Dihidroksi-2-butanon-4-fosfatna sintaza
(Preusmjereno sa stranice 3,4-dihidroksi-2-butanon-4-fosfatna sintaza)
| 3,4-Dihidroksi-2-butanon-4-fosfatna sintaza | |||||||||
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| Identifikatori | |||||||||
| EC broj | 4.1.99.12 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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3,4-Dihidroksi-2-butanon-4-fosfatna sintaza (EC 4.1.99.12, DHBP sintaza, L-3,4-dihidroksibutan-2-on-4-fosfat sintaza) je enzim sa sistematskim imenom D-ribuloza 5-fosfat format-lijaza (formira L-3,4-dihidroksibutan-2-on 4-fosfat).[1][2][3][4][5][6][7][8] Ovaj enzim katalizuje sledeću hemijsku reakciju
- D-ribuloza 5-fosfat format + L-3,4-dihidroksibutan-2-on 4-fosfat
Za dejstvo ovog enzima je neophodan divalentni katjon, preferentno Mg2+.
Reference[uredi | uredi kod]
- ↑ Volk, R. and Bacher, A. (1990). „Studies on the 4-carbon precursor in the biosynthesis of riboflavin. Purification and properties of L-3,4-dihydroxy-2-butanone-4-phosphate synthase”. J. Biol. Chem. 265: 19479-19485. PMID 2246238.
- ↑ Liao, D.I., Calabrese, J.C., Wawrzak, Z., Viitanen, P.V. and Jordan, D.B. (2001). „Crystal structure of 3,4-dihydroxy-2-butanone 4-phosphate synthase of riboflavin biosynthesis”. Structure 9: 11-18. PMID 11342130.
- ↑ Kelly, M.J., Ball, L.J., Krieger, C., Yu, Y., Fischer, M., Schiffmann, S., Schmieder, P., Kühne, R., Bermel, W., Bacher, A., Richter, G. and Oschkinat, H. (2001). „The NMR structure of the 47-kDa dimeric enzyme 3,4-dihydroxy-2-butanone-4-phosphate synthase and ligand binding studies reveal the location of the active site”. Proc. Natl. Acad. Sci. USA 98: 13025-13030. PMID 11687623.
- ↑ Liao, D.I., Zheng, Y.J., Viitanen, P.V. and Jordan, D.B. (2002). „Structural definition of the active site and catalytic mechanism of 3,4-dihydroxy-2-butanone-4-phosphate synthase”. Biochemistry 41: 1795-1806. PMID 11827524.
- ↑ Fischer, M., Römisch, W., Schiffmann, S., Kelly, M., Oschkinat, H., Steinbacher, S., Huber, R., Eisenreich, W., Richter, G. and Bacher, A. (2002). „Biosynthesis of riboflavin in archaea studies on the mechanism of 3,4-dihydroxy-2-butanone-4-phosphate synthase of Methanococcus jannaschii”. J. Biol. Chem. 277: 41410-41416. PMID 12200440.
- ↑ Steinbacher, S., Schiffmann, S., Richter, G., Huber, R., Bacher, A. and Fischer, M. (2003). „Structure of 3,4-dihydroxy-2-butanone 4-phosphate synthase from Methanococcus jannaschii in complex with divalent metal ions and the substrate ribulose 5-phosphate: implications for the catalytic mechanism”. J. Biol. Chem. 278: 42256-42265. PMID 12904291.
- ↑ Steinbacher, S., Schiffmann, S., Bacher, A. and Fischer, M. (2004). „Metal sites in 3,4-dihydroxy-2-butanone 4-phosphate synthase from Methanococcus jannaschii in complex with the substrate ribulose 5-phosphate”. Acta Crystallogr. D Biol. Crystallogr. 60: 1338-1340. PMID 15213409.
- ↑ Echt, S., Bauer, S., Steinbacher, S., Huber, R., Bacher, A. and Fischer, M. (2004). „Potential anti-infective targets in pathogenic yeasts: structure and properties of 3,4-dihydroxy-2-butanone 4-phosphate synthase of Candida albicans”. J. Mol. Biol. 341: 1085-1096. PMID 15328619.
Literatura[uredi | uredi kod]
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.