2-Halokiselinska dehalogenaza (invertovanje konfiguracije)

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2-Halokiselinska dehalogenaza (invertovanje konfiguracije)
Identifikatori
EC broj 3.8.1.10
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures

2-Halokiselinska dehalogenaza (invertovanje konfiguracije) (EC 3.8.1.10, 2-haloalkanoidno kiselinska dehalogenaza, 2-haloalkanoidno kiselinska halidohidrolaza, DL-2-halokiselinska dehalogenaza, DL-2-halokiselinska dehalogenaza (inverzija konfiguracije), DL-2-halokiselinska halidohidrolaza (inverzija konfiguracije), DL-DEXi, (R,S)-2-halokiselinska dehalogenaza (inverzija konfiguracije)) je enzim sa sistematskim imenom (S)-2-halokiselina dehalogenaza (inverzija konfiguracije).[1][2][3][4][5][6][7][8][9] Ovaj enzim katalizuje sledeću hemijsku reakciju

(1) (S)-2-halokiselina + H2O (R)-2-hidroksikiselina + halid
(2) (R)-2-halokiselina + H2O (S)-2-hidroksikiselina + halid

Ovaj enzim dehalogenuje (S)- i (R)-2-haloalkanoinske kiseline do korespondirajućih (R)- i (S)-hidroksialkanoinskih kiselina.

Reference[uredi - уреди | uredi izvor]

  1. Motosugi, K., Esahi, N. and Soda, K. (1982). "Bacterial assimilation of D- and L-2-chloropropionates and occurrence of a new dehalogenase". Arch. Microbiol. 131: 179–183. PMID 7103659. 
  2. Motosugi, K., Esahi, N. and Soda, K. Purification and properties of a new enzyme, DL- (1982). "2-haloacid dehalogenase, from Pseudomonas sp". J. Bacteriol. 150: 522–527. PMID 7068529. 
  3. Motosugi, K., Esahi, N. and Soda, K. (1984). "Enzymatic preparation of D- and L-lactic acid from racemic 2-chloropropionic acid". Biotechnol. Bioeng. 26: 805–806. PMID 18889931. 
  4. Kurihara, T., Esaki, N. and Soda, K. (2000). "Bacterial 2-haloacid dehalogenases: structures and reaction mechanisms". J. Mol. Catal., B Enzym. 10: 57–65. 
  5. Liu, J.-Q., Kurihara, T., Hasan, A.K.M.Q., Nardi-Dei, V., Koshikawa, H., Esaki, N. and Soda, K. (1994). "Purification and characterization of thermostable and nonthermostable 2-haloacid dehalogenases with different stereospecificities from Pseudomonas sp. strain YL". Appl. Environ. Microbiol. 60: 2389–2393. PMID 8074519. 
  6. Cairns, S.S., Cornish, A. and Cooper, R.A. (1996). "Cloning, sequencing and expression in Escherichia coli of two Rhizobium sp. genes encoding haloalkanoate dehalogenases of opposite stereospecificity". Eur. J. Biochem. 235: 744–749. PMID 8654424. 
  7. Leigh, J.A., Skinner, A.J. and Cooper, R.A. (1988). "Partial purification, stereospecificity and stoichiometry of three dehalogenases from a Rhizobium species". FEMS Microbiol. Lett. 49: 353–356. 
  8. Weightman, A.J., Weightman, A.L. and Slater, J.H. (1982). "Stereospecificity of 2-monochloropropionate dehalogenation by the two dehalogenases of Pseudomonas P3: evidence for two different dehalogenation mechanisms". J. Gen. Microbiol. 128: 1755–1762. PMID 7142958. 
  9. Soda, K., Kurihara, T., Liu, J.-Q., Nardi-Dei, V., Park, C., Miyagi, M., Tsunasawa, S. and Esaki, N. (1996). "Bacterial 2-haloacid dehalogenases: Structures and catalytic properties". Pure Appl. Chem. 68: 2097–2103. 

Literatura[uredi - уреди | uredi izvor]

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