2-Oksoglutarat sintaza
| 2-Oksoglutarat sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.2.7.3 | ||||||||
| CAS broj | 37251-05-1 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
2-Oksoglutarat sintaza (EC 1.2.7.3, 2-ketoglutarat feredoksinska oksidoreduktaza, 2-oksoglutarat:feredoksin oksidoreduktaza, KGOR, 2-oksoglutarat feredoksinska oksidoreduktaza, 2-oksoglutarat:feredoksin 2-oksidoreduktaza (KoA-sukcinilacija)) je enzim sa sistematskim imenom 2-oksoglutarat:feredoksin oksidoreduktaza (dekarboksilacija).[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju
- 2-oksoglutarat + KoA + 2 oksidovani feredoksin sukcinil-KoA + CO2 + 2 redukovani feredoksin + 2 H+
Ovaj enzim je jedan od četiri 2-oksokiselinske oksidoreduktaze koje se razlikuju po njihovoj sposobnosti da oksidativno dekarboksilišu različite 2-oksokiseline i formiraju njihove KoA derivate, cf. EC 1.2.7.1, piruvat sintaza, EC 1.2.7.7, 3-metil-2-oksobutanoat dehidrogenaza (feredoksin) i EC 1.2.7.8, indolpiruvat feredoksin oksidoreduktaza. Ovaj enzim sadrži tiamin difosfat i 2 [4Fe-4S] klastere. On je visoko specifičan za 2-oksoglutarat.
Reference[uredi | uredi kod]
- ↑ Buchanan, B.B. and Evans, M.C.W. (1965). „The synthesis of α-ketoglutarate from succinate and carbon dioxide by a subcellular preparation of a photosynthetic bacterium”. Proc. Natl. Acad. Sci. USA 54: 1212-1218. PMID 4286833.
- ↑ Gehring, U. and Arnon, D.I. (1972). „Purification and properties of α-ketoglutarate synthase from a photosynthetic bacterium”. J. Biol. Chem. 247: 6963-6969. PMID 4628267.
- ↑ Dorner, E. and Boll, M. (2002). „Properties of 2-oxoglutarate:ferredoxin oxidoreductase from Thauera aromatica and its role in enzymatic reduction of the aromatic ring”. J. Bacteriol. 184: 3975-3983. PMID 12081970.
- ↑ Mai, X. and Adams, M.W. (1996). „Characterization of a fourth type of 2-keto acid-oxidizing enzyme from a hyperthermophilic archaeon: 2-ketoglutarate ferredoxin oxidoreductase from Thermococcus litoralis”. J. Bacteriol. 178: 5890-5896. PMID 8830683.
- ↑ Schut, G.J., Menon, A.L. and Adams, M.W.W. (2001). „2-Keto acid oxidoreductases from Pyrococcus furiosus and Thermococcus litoralis”. Methods Enzymol. 331: 144-158. PMID 11265457.
Literatura[uredi | uredi kod]
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.