16S rRNK (adenin1518-N6/adenin1519-N6)-dimetiltransferaza

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16S rRNK (adenin1518-N6/adenin1519-N6)-dimetiltransferaza
Identifikatori
EC broj 2.1.1.182
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB RCSB PDB PDBe PDBj PDBsum

16S rRNK (adenin1518-N6/adenin1519-N6)-dimetiltransferaza (EC 2.1.1.182, S-adenozilmetionin-6-N',N'-adenozil (rRNK) dimetiltransferaza, KsgA, ksgA metiltransferaza) je enzim sa sistematskim imenom S-adenozil-L-metionin:16S rRNK (adenin1518-N6/adenin1519-N6)-dimetiltransferaza.[1][2][3][4][5][6][7][8] Ovaj enzim katalizuje sledeću hemijsku reakciju

4 S-adenozil-L-metionin + adenin1518/adenin1519 u 16S rRNK 4 S-adenozil-L-homocistein + N6-dimetiladenin1518/N6-dimetiladenin1519 u 16S rRNK

KsgA proizvodi modifikaciju ribozomalne RNK koja je u najvećoj meri konzervirana, dimetilaciju adenina1518 i adenina1519 u 16S rRNK. Vrste koje nemaju ovu metilazu su otporne na kasugamicin.

Reference[uredi | uredi kod]

  1. Helser, T.L., Davies, J.E. and Dahlberg, J.E. (1971). „Change in methylation of 16S ribosomal RNA associated with mutation to kasugamycin resistance in Escherichia coli”. Nat. New Biol. 233: 12-14. PMID 4329247. 
  2. Helser, T.L., Davies, J.E. and Dahlberg, J.E. (1972). „Mechanism of kasugamycin resistance in Escherichia coli”. Nat. New Biol. 235: 6-9. PMID 4336392. 
  3. van Buul, C.P. and van Knippenberg, P.H. (1985). „Nucleotide sequence of the ksgA gene of Escherichia coli: comparison of methyltransferases effecting dimethylation of adenosine in ribosomal RNA”. Gene 38: 65-72. PMID 3905517. 
  4. Formenoy, L.J., Cunningham, P.R., Nurse, K., Pleij, C.W. and Ofengand, J. (1994). „Methylation of the conserved A1518-A1519 in Escherichia coli 16S ribosomal RNA by the ksgA methyltransferase is influenced by methylations around the similarly conserved U1512.G1523 base pair in the 3′ terminal hairpin”. Biochimie 76: 1123-1128. PMID 7538324. 
  5. O'Farrell, H.C., Scarsdale, J.N. and Rife, J.P. (2004). „Crystal structure of KsgA, a universally conserved rRNA adenine dimethyltransferase in Escherichia coli”. J. Mol. Biol. 339: 337-353. PMID 15136037. 
  6. Poldermans, B., Roza, L. and Van Knippenberg, P.H. (1979). „Studies on the function of two adjacent N6,N6-dimethyladenosines near the 3′ end of 16 S ribosomal RNA of Escherichia coli. III. Purification and properties of the methylating enzyme and methylase-30 S interactions”. J. Biol. Chem. 254: 9094-9100. PMID 383712. 
  7. Demirci, H., Belardinelli, R., Seri, E., Gregory, S.T., Gualerzi, C., Dahlberg, A.E. and Jogl, G. (2009). „Structural rearrangements in the active site of the Thermus thermophilus 16S rRNA methyltransferase KsgA in a binary complex with 5′-methylthioadenosine”. J. Mol. Biol. 388: 271-282. PMID 19285505. 
  8. Tu, C., Tropea, J.E., Austin, B.P., Court, D.L., Waugh, D.S. and Ji, X. (2009). „Structural basis for binding of RNA and cofactor by a KsgA methyltransferase”. Structure 17: 374-385. PMID 19278652. 

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