15-Cis-fitoen desaturaza
(Preusmjereno sa stranice 15-cis-phytoene:plastoquinone oxidoreductase)
| 15-Cis-fitoen desaturaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.3.5.5 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
15-Cis-fitoen desaturaza (EC 1.3.5.5, fitoenska desaturaza (nespecifična), PDS) je enzim sa sistematskim imenom 15-cis-fitoen:plastohinon oksidoreduktaza.[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
- 15-cis-fitoen + 2 plastohinon 9,15,9'-tricis-zeta-karoten + 2 plastohinol (sveukupna reakcija)
(1a) 15-cis-fitoen + plastohinon 15,9'-dicis-fitofluen + plastohinol (1b) 15,9'-dicis-fitofluen + plastohinon 9,15,9'-tricis-zeta-karoten + plastohinol
This enzim is involved in biosintezu karotenoida kod biljki i cijanobakterija. Enzim iz Synechococcus can takođe koristi NAD+ i NADP+ kao elektronske akceptore pod anaerobnim uslovima. Enzim iz Gentiana lutea nije aktivan na NAD+ i NADP+.
Reference[uredi | uredi kod]
- ↑ Breitenbach, J., Zhu, C. and Sandmann, G. (2001). „Bleaching herbicide norflurazon inhibits phytoene desaturase by competition with the cofactors”. J. Agric. Food Chem. 49: 5270-5272. PMID 11714315.
- ↑ Schneider, C., Boger, P. and Sandmann, G. (1997). „Phytoene desaturase: heterologous expression in an active state, purification, and biochemical properties”. Protein Expr. Purif. 10: 175-179. PMID 9226712.
- ↑ Fraser, P.D., Linden, H. and Sandmann, G. (1993). „Purification and reactivation of recombinant Synechococcus phytoene desaturase from an overexpressing strain of Escherichia coli”. Biochem. J. 291: 687-692. PMID 8489496.
- ↑ Breitenbach, J. and Sandmann, G. (2005). „ζ-Carotene cis isomers as products and substrates in the plant poly-cis carotenoid biosynthetic pathway to lycopene”. Planta 220: 785-793. PMID 15503129.
Literatura[uredi | uredi kod]
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.