Pseudomonalizin
(Preusmjereno sa stranice Pseudomonas sp. pepstatin-insenzitivna karboksilna proteinaza)
Pseudomonalizin | |||||||||
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Identifikatori | |||||||||
EC broj | 3.4.21.100 | ||||||||
CAS broj | 848318-58-1 | ||||||||
IntEnz | IntEnz view | ||||||||
BRENDA | BRENDA entry | ||||||||
ExPASy | NiceZyme view | ||||||||
KEGG | KEGG entry | ||||||||
MetaCyc | metabolic pathway | ||||||||
PRIAM | profile | ||||||||
PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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Pseudomonalizin (EC 3.4.21.100, Pseudomonas sp. pepstatin-insenzitivna karboksilna proteinaza, pseudomonapepsin, sedolizin) je enzim.[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
- Hidroliza B lanca insulina na -Glu13-Ala-, -Leu15-Tyr- i -Phe25-Tyr-, i angiotenzina I na -Tyr4-Ile-. Dobar sintetički supstrat je Lys-Pro-Ile-Glu-Phe-Phe(NO2)-Arg-Leu.
Ovaj enzim izlučuje Pseudomonas sp. No. 101. Optimalni pH je 4.
- ↑ Oda, K., Sugitani, M., Fukuhara, K. and Murao, S. (1987). „Purification and properties of a pepstatin-insensitive carboxyl proteinase from a Gram-negative bacterium”. Biochim. Biophys. Acta 923: 463-469. PMID 3548827.
- ↑ Oda, K., Nakatani, H. and Dunn, B.M. (1992). „Substrate specificity and kinetic properties of pepstatin-insensitive carboxyl proteinase from Pseudomonas sp. No. 101”. Biochim. Biophys. Acta 1120: 208-214. PMID 1562589.
- ↑ Wlodawer, A., Li, M., Dauter, Z., Gustchina, A., Uchida, K., Oyama, H., Dunn, B.M. and Oda, K. (2001). „Carboxyl proteinase from Pseudomonas defines a novel family of subtilisin-like enzymes”. Nat. Struct. Biol. 8: 442-446. PMID 11323721.
- ↑ Wlodawer, A., Li, M., Gustchina, A., Oyama, H., Dunn, B.M. and Oda, K. (2003). „Structural and enzymatic properties of the sedolisin family of serine-carboxyl peptidases”. Acta Biochim. Pol. 50: 81-102. PMID 12673349.
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