Latosterol oksidaza
(Preusmjereno sa stranice 5-DES)
Latosterol oksidaza | |||||||||
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Identifikatori | |||||||||
EC broj | 1.14.21.6 | ||||||||
CAS broj | 37255-37-1 | ||||||||
IntEnz | IntEnz view | ||||||||
BRENDA | BRENDA entry | ||||||||
ExPASy | NiceZyme view | ||||||||
KEGG | KEGG entry | ||||||||
MetaCyc | metabolic pathway | ||||||||
PRIAM | profile | ||||||||
PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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Latosterol oksidaza (EC 1.14.21.6, Delta7-sterol Delta5-dehidrogenaza, Delta7-sterol 5-desaturaza, Delta7-sterol-C5(6)-desaturaza, 5-DES) je enzim sa sistematskim imenom 5alfa-holest-7-en-3beta-ol,NAD(P)H:kiseonik 5-oksidoreduktaza.[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
- 5alfa-holest-7-en-3beta-ol + NAD(P)H + H+ + O2 holesta-5,7-dien-3beta-ol + NAD(P)+ + 2H2O
Ovaj enzim katalizuje uvođenje C5 dvostruke veze u B prsten Delta7-sterola čime nastaju korespondirajući Delta5,7-steroli kod sisara, kvasca i biljki.
- ↑ Dempsey, M.E., Seaton, J.D., Schroepfer, G.J. and Trockman, R.W. (1964). „The intermediary role of Δ5,7-cholestadien-3β-ol in cholesterol biosynthesis”. J. Biol. Chem. 239: 1381-1387. PMID 14189869.
- ↑ Nishino, H., Nakaya, J., Nishi, S., Kurosawa, T. and Ishibashi, T. (1997). „Temperature-induced differential kinetic properties between an initial burst and the following steady state in membrane-bound enzymes: studies on lathosterol 5-desaturase”. Arch. Biochem. Biophys. 339: 298-304. PMID 9056262.
- ↑ Taton, M. and Rahier, A. (1996). „Plant sterol biosynthesis: identification and characterization of higher plant Δ7-sterol C5(6)-desaturase”. Arch. Biochem. Biophys. 325: 279-288. PMID 8561508.
- ↑ Taton, M., Husselstein, T., Benveniste, P. and Rahier, A. (2000). „Role of highly conserved residues in the reaction catalyzed by recombinant Δ7-sterol-C5(6)-desaturase studied by site-directed mutagenesis”. Biochemistry 39: 701-711. PMID 10651635.
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