Lipid IVA 4-amino-4-dezoksi-L-arabinoziltransferaza

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Lipid IVA 4-amino-4-dezoksi-L-arabinoziltransferaza
Identifikatori
EC broj 2.4.2.43
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures

Lipid IVA 4-amino-4-dezoksi-L-arabinoziltransferaza (EC 2.4.2.43, undekaprenil fosfat-alfa-L-Ara4N transferaza, 4-amino-4-dezoksi-L-arabinoza lipid A transferaza, protein polimiksinske otpornosti PmrK, arnT (gen)) je enzim sa sistematskim imenom 4-amino-4-dezoksi-alfa-L-arabinopiranozil ditran,oktacis-undekaprenil fosfat:lipid IVA 4-amino-4-dezoksi-L-arabinopiranosiltransferaza.[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju

(1) 4-amino-4-dezoksi-alfa-L-arabinopiranozil ditrans,oktacis-undekaprenil fosfat + alfa-Kdo-(2->4)-alfa-Kdo-(2->6)-lipid A \rightleftharpoons alfa-Kdo-(2->4)-alfa-Kdo-(2->6)-[4-P-L-Ara4N]-lipid A + ditrans,oktacis-undekaprenil fosfat
(2) 4-amino-4-dezoksi-alfa-L-arabinopiranozil ditrans,oktacis-undekaprenil fosfat + lipid IVA \rightleftharpoons lipid IIA + ditrans,oktacis-undekaprenil fosfat
(3) 4-amino-4-dezoksi-alfa-L-arabinopiranozil ditrans,oktacis-undekaprenil fosfat + alfa-Kdo-(2->4)-alfa-Kdo-(2->6)-lipid IVA \rightleftharpoons 4'-alfa-L-Ara4N-alfa-Kdo-(2->4)-alfa-Kdo-(2->6)-lipid IVA + ditrans,oktacis-undekaprenil fosfat

Ovaj integralni membranski protein je prisutan na unutrašnjim membranama pojedinih Gram negativnih endobakterija.

Reference[uredi - уреди]

  1. Trent, M.S., Ribeiro, A.A., Lin, S., Cotter, R.J. and Raetz, C.R. (2001). "An inner membrane enzyme in Salmonella and Escherichia coli that transfers 4-amino-4-deoxy-L-arabinose to lipid A: induction on polymyxin-resistant mutants and role of a novel lipid-linked donor". J. Biol. Chem. 276: 43122–43131. PMID 11535604. 
  2. Trent, M.S., Ribeiro, A.A., Doerrler, W.T., Lin, S., Cotter, R.J. and Raetz, C.R. (2001). "Accumulation of a polyisoprene-linked amino sugar in polymyxin-resistant Salmonella typhimurium and Escherichia coli: structural characterization and transfer to lipid A in the periplasm". J. Biol. Chem. 276: 43132–43144. PMID 11535605. 
  3. Zhou, Z., Ribeiro, A.A., Lin, S., Cotter, R.J., Miller, S.I. and Raetz, C.R. (2001). "Lipid A modifications in polymyxin-resistant Salmonella typhimurium: PMRA-dependent 4-amino-4-deoxy-L-arabinose, and phosphoethanolamine incorporation". J. Biol. Chem. 276: 43111–43121. PMID 11535603. 
  4. Bretscher, L.E., Morrell, M.T., Funk, A.L. and Klug, C.S. (2006). "Purification and characterization of the L-Ara4N transferase protein ArnT from Salmonella typhimurium". Protein Expr. Purif. 46: 33–39. PMID 16226890. 
  5. Impellitteri, N.A., Merten, J.A., Bretscher, L.E. and Klug, C.S. (2010). "Identification of a functionally important loop in Salmonella typhimurium ArnT". Biochemistry 49: 29–35. PMID 19947657. 

Literatura[uredi - уреди]

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