L-cistein:1D-mio-inozitol 2-amino-2-dezoksi-alfa-D-glukopiranozidna ligaza
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| „L“-cistein:1D-mio-inozitol 2-amino-2-dezoksi-alfa-D-glukopiranozidna ligaza | |||||||||
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| Identifikatori | |||||||||
| EC broj | 6.3.1.13 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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L-cistein:1D-mio-inozitol 2-amino-2-dezoksi-alfa-D-glukopiranozidna ligaza (EC 6.3.1.13, MshC, MshC ligaza, Cys:GlcN-Ins ligaza, mikotiolna ligaza) je enzim sa sistematskim imenom L-cistein:1-O-(2-amino-2-dezoksi-alfa-D-glukopiranosil)-1D-mio-inozitol ligaza (formira AMP).[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
- 1-O-(2-amino-2-dezoksi-alfa-D-glukopiranozil)-1D-mio-inozitol + L-cistein + ATP 1-O-[2-(L-cisteinamido)-2-dezoksi-alfa-D-glukopiranozil]-1D-mio-inozitol + AMP + difosfat
Ovaj enzim je ključan za biosintezu mikotiola.
Reference[uredi | uredi kod]
- ↑ Fan, F., Luxenburger, A., Painter, G.F. and Blanchard, J.S. (2007). „Steady-state and pre-steady-state kinetic analysis of Mycobacterium smegmatis cysteine ligase (MshC)”. Biochemistry 46: 11421-11429. PMID 17848100.
- ↑ Gutierrez-Lugo, M.T., Newton, G.L., Fahey, R.C. and Bewley, C.A. (2006). „Cloning, expression and rapid purification of active recombinant mycothiol ligase as B1 immunoglobulin binding domain of streptococcal protein G, glutathione-S-transferase and maltose binding protein fusion proteins in Mycobacterium smegmatis”. Protein Expr. Purif. 50: 128-136. PMID 16908186.
- ↑ Tremblay, L.W., Fan, F., Vetting, M.W. and Blanchard, J.S. (2008). „The 1.6 Å crystal structure of Mycobacterium smegmatis MshC: the penultimate enzyme in the mycothiol biosynthetic pathway”. Biochemistry 47: 13326-13335. PMID 19053270.
Literatura[uredi | uredi kod]
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.