Kaspaza-3

Izvor: Wikipedia
Kaspaza-3
Caspase3 1rhk.png
Identifikatori
EC broj 3.4.22.56
CAS broj 169592-56-7
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures

Kaspaza-3 (EC 3.4.22.56, CPP32, apopain, jama protein) je enzim.[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju

Neophodno je prisustvo Asp ostatka u pozicijama P1 i P4. Preferentno dolazi do razlaganja sekvence Asp-Xaa-Xaa-Asp- sa hidrofobnim aminokiselinskim ostatkom u P2 poziciji i hidrofilnim aminokiselinskim ostatkom u P3, mada Val ili Ala takođe mogu da budu u toj poziciji

Kaspaza-3 je efektor/izvršilac kaspaze, kao što su i kaspaza-6 (EC 3.4.22.59) i kaspaza-7 (EC 3.4.22.60).

Reference[uredi - уреди]

  1. Krebs, J.F., Srinivasan, A., Wong, A.M., Tomaselli, K.J., Fritz, L.C. and Wu, J.C. (2000). "Heavy membrane-associated caspase 3: identification, isolation, and characterization". Biochemistry 39: 16056–16063. PMID 11123933. 
  2. Li, H., Bergeron, L., Cryns, V., Pasternack, M.S., Zhu, H., Shi, L., Greenberg, A. and Yuan, J. (1997). "Activation of caspase-2 in apoptosis". J. Biol. Chem. 272: 21010–21017. PMID 9261102. 
  3. Nicholson, D. and Thornberry, N.A. (2004). "Caspase-3 and caspase-7". u: Barrett, A.J., Rawlings, N.D. and Woessner, J.F.. Handbook of Proteolytic Enzymes (2nd izd.). London: Elsevier. str. 1298-1302. 
  4. Fang, B., Boross, P.I., Tozser, J. and Weber, I.T. (2006). "Structural and kinetic analysis of caspase-3 reveals role for S5 binding site in substrate recognition". J. Mol. Biol. 360: 654–666. PMID 16781734. 
  5. Chang, H.Y. and Yang, X. (2000). "Proteases for cell suicide: functions and regulation of caspases". Microbiol. Mol. Biol. Rev. 64: 821–846. PMID 11104820. 
  6. Martin, S.J., Amarante-Mendes, G.P., Shi, L., Chuang, T.H., Casiano, C.A., O'Brien, G.A., Fitzgerald, P., Tan, E.M., Bokoch, G.M., Greenberg, A.H. and Green, D.R. (1996). "The cytotoxic cell protease granzyme B initiates apoptosis in a cell-free system by proteolytic processing and activation of the ICE/CED-3 family protease, CPP32, via a novel two-step mechanism". EMBO J. 15: 2407–2416. PMID 8665848. 

Literatura[uredi - уреди]

  • Nicholson, D. and Thornberry, N.A. (2004). "Caspase-3 and caspase-7". u: Barrett, A.J., Rawlings, N.D. and Woessner, J.F.. Handbook of Proteolytic Enzymes (2nd izd.). London: Elsevier. str. 1298-1302. 

Spoljašnje veze[uredi - уреди]