Glikopeptidna alfa-N-acetilgalaktozaminidaza

Izvor: Wikipedia
Glikopeptidna alfa-N-acetilgalaktozaminidaza
Identifikatori
EC broj 3.2.1.97
CAS broj 59793-96-3
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures

Glikopeptidna alfa-N-acetilgalaktozaminidaza (EC 3.2.1.97, endo-alfa-acetilgalaktozaminidaza, endo-alfa-N-acetil-D-galaktozaminidaza, mucinaminilserin mucinaminidaza, D-galaktozil-3-(N-acetil-alfa-D-galaktozaminil)-L-serin mucinaminohidrolaza, endo-alfa-GalNAc-aza, glikopeptid alfa-N-acetilgalaktozaminidaza, D-galaktozil-N-acetil-alfa-D-galaktozamin D-galaktozil-N-acetil-galaktozaminohidrolaza) je enzim sa sistematskim imenom glikopeptid-D-galaktozil-N-acetil-alfa-D-galaktozamin D-galaktozil-N-acetil-galaktozaminohidrolaza.[1][2][3][4][5][6][7] Ovaj enzim katalizuje sledeću hemijsku reakciju

3-O-beta-D-galaktozil-N-acetil-alfa-D-galaktozaminil-L-serin-[protein] + H2O \rightleftharpoons 3-O-beta-D-galaktozil-N-acetil-alfa-D-galaktozamin + L-serin-[protein]

Ovaj enzim katalizuje odvajanje Gal-(1->3)-beta-GalNAc alfa-vezanog za ostatke serina ili treonina u glikoproteinima mucinskog-tipa.

Reference[uredi - уреди]

  1. Ashida, H., Maki, R., Ozawa, H., Tani, Y., Kiyohara, M., Fujita, M., Imamura, A., Ishida, H., Kiso, M. and Yamamoto, K. (2008). "Characterization of two different endo-α-N-acetylgalactosaminidases from probiotic and pathogenic enterobacteria, Bifidobacterium longum and Clostridium perfringens". Glycobiology 18: 727–734. PMID 18559962. 
  2. Koutsioulis, D., Landry, D. and Guthrie, E.P. (2008). "Novel endo-α-N-acetylgalactosaminidases with broader substrate specificity". Glycobiology 18: 799–805. PMID 18635885. 
  3. Fujita, K., Oura, F., Nagamine, N., Katayama, T., Hiratake, J., Sakata, K., Kumagai, H. and Yamamoto, K. (2005). "Identification and molecular cloning of a novel glycoside hydrolase family of core 1 type O-glycan-specific endo-α-N-acetylgalactosaminidase from Bifidobacterium longum". J. Biol. Chem. 280: 37415–37422. PMID 16141207. 
  4. Suzuki, R., Katayama, T., Kitaoka, M., Kumagai, H., Wakagi, T., Shoun, H., Ashida, H., Yamamoto, K. and Fushinobu, S. (2009). "Crystallographic and mutational analyses of substrate recognition of endo-α-N-acetylgalactosaminidase from Bifidobacterium longum". J. Biochem. 146: 389–398. PMID 19502354. 
  5. Gregg, K.J. and Boraston, A.B. (2009). "Cloning, recombinant production, crystallization and preliminary X-ray diffraction analysis of a family 101 glycoside hydrolase from Streptococcus pneumoniae". Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 65: 133–135. PMID 19194003. 
  6. Ashida, H., Yamamoto, K., Murata, T., Usui, T. and Kumagai, H. (2000). "Characterization of endo-α-N-acetylgalactosaminidase from Bacillus sp. and syntheses of neo-oligosaccharides using its transglycosylation activity". Arch. Biochem. Biophys. 373: 394–400. PMID 10620364. 
  7. Goda, H.M., Ushigusa, K., Ito, H., Okino, N., Narimatsu, H. and Ito, M. (2008). "Molecular cloning, expression, and characterization of a novel endo-α-N-acetylgalactosaminidase from Enterococcus faecalis". Biochem. Biophys. Res. Commun. 375: 441–446. PMID 18725192. 

Literatura[uredi - уреди]

Spoljašnje veze[uredi - уреди]