Izocitrat dehidrogenaza (NAD+)
| Izocitrat dehidrogenaza (NAD+) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
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| Dimer izocitrat dehidrogenaze (čovjek) | |||||||||
| Identifikatori | |||||||||
| EC broj | 1.1.1.41 | ||||||||
| CAS broj | 9001-58-5 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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Izocitrat dehidrogenaza (NAD+) (EC 1.1.1.41, beta-ketoglutarinska-izocitratna karboksilaza, izocitratna kiselina dehidrogenaza, NAD zavisna izocitratna dehidrogenaza, NAD izocitratna dehidrogenaza, NAD-vezana izocitratna dehidrogenaza, NAD-specifična izocitratna dehidrogenaza, NAD izocitratna dehidrogenaza, izocitratna dehidrogenaza (NAD), nikotinamid adenin dinukleotid izocitratna dehidrogenaza) je enzim sa sistematskim imenom izocitrat:NAD+ oksidoreduktaza (dekarboksilacija).[1][2][3][4][5][6][7][8][9] Ovaj enzim katalizuje sledeću hemijsku reakciju
- izocitrat + NAD+ 2-oksoglutarat + CO2 + NADH
Neophodni su Mn2+ ili Mg2+ za aktivnost. Za razliku od EC 1.1.1.42, izocitratna dehidrogenaza (NADP+), ne može da koristi oksalosukcinat kao supstrat. Kod eukariota, izocitratna dehidrogenaza postoji u dve forme: NAD+-vezani enzim koji je prisutan u mitohondrijama i ima alosterna svojstva, i nealosterni, NADP+-vezani enzim koji je prisutan u mitohondrijama i u citoplazmi.
Reference[uredi | uredi kod]
- ↑ Hathaway, J.A. and Atkinson, D.E. (1963). „The effect of adenylic acid on yeast nicotinamide adenine dinucleotide isocitrate dehydrogenase, a possible metabolic control mechanism”. J. Biol. Chem. 238: 2875-2881. PMID 14063317.
- ↑ Kornberg, A. and Pricer, W.E. (1951). „Di- and triphosphopyridine nucleotide isocitric dehydrogenase in yeast”. J. Biol. Chem. 189: 123-136. PMID 14832224.
- ↑ Plaut, G.W.E. (1963). „Isocitrate dehydrogenases”. u: Boyer, P.D., Lardy, H. and Myrbäck, K.. The Enzymes. 7 (2nd izd.). New York: Academic Press. str. 105-126.
- ↑ Plaut, G.W.E. and Sung, S.-C. (1954). „Diphosphopyridine nucleotide isocitric dehydrogenase from animal tissues”. J. Biol. Chem. 207: 305-314. PMID 13152105.
- ↑ Ramakrishnan, C.V. and Martin, S.M. (1955). „Isocitric dehydrogenase in Aspergillus niger”. Arch. Biochem. Biophys. 55: 403-407.
- ↑ Vickery, H.B. (1962). „A suggested new nomenclature for the isomers of isocitric acid”. J. Biol. Chem. 237: 1739-1741. PMID 13925783.
- ↑ Camacho, M.L., Brown, R.A., Bonete, M.J., Danson, M.J. and Hough, D.W. (1995). „Isocitrate dehydrogenases from Haloferax volcanii and Sulfolobus solfataricus: enzyme purification, characterisation and N-terminal sequence”. FEMS Microbiol. Lett. 134: 85-90. PMID 8593959.
- ↑ Kim, Y.O., Koh, H.J., Kim, S.H., Jo, S.H., Huh, J.W., Jeong, K.S., Lee, I.J., Song, B.J. and Huh, T.L. (1999). „Identification and functional characterization of a novel, tissue-specific NAD+-dependent isocitrate dehydrogenase β subunit isoform”. J. Biol. Chem. 274: 36866-36875. PMID 10601238.
- ↑ Inoue, H., Tamura, T., Ehara, N., Nishito, A., Nakayama, Y., Maekawa, M., Imada, K., Tanaka, H. and Inagaki, K. (2002). „Biochemical and molecular characterization of the NAD+-dependent isocitrate dehydrogenase from the chemolithotroph Acidithiobacillus thiooxidans”. FEMS Microbiol. Lett. 214: 127-132. PMID 12204383.
Literatura[uredi | uredi kod]
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.