Deacetoksicefalosporin-C hidroksilaza
Prijeđi na navigaciju
Prijeđi na pretragu
| Deacetoksicefalosporin-C hidroksilaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.11.26 | ||||||||
| CAS broj | 69772-89-0 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Deacetoksicefalosporin-C hidroksilaza (EC 1.14.11.26, deacetilcefalosporinska C sintaza, 3'-metilcefemska hidroksilaza, DACS, DAOC hidroksilaza, deacetoksicefalosporinska C hidroksilaza) je enzim sa sistematskim imenom deacetoksicephalosporin-C,2-oksoglutarat:kiseonik oksidoreduktaza (3-hidroksilacija).[1][2][3][4][5][6][7] Ovaj enzim katalizuje sledeću hemijsku reakciju
- deacetoksicefalosporin C + 2-oksoglutarat + O2 deacetilcefalosporin C + sukcinat + CO2
Za rad ovog enzima je neophodan Fe2+ jon. Enzim takođe može da koristi 3-eksometilincefalosporin C kao supstrat da formira deacetoksicefalosporin C.
Reference[uredi | uredi kod]
- ↑ Dotzlaf, J.E. and Yeh, W.K. (1987). „Copurification and characterization of deacetoxycephalosporin C synthetase/hydroxylase from Cephalosporium acremonium”. J. Bacteriol. 169: 1611-1618. PMID 3558321.
- ↑ Baker, B.J., Dotzlaf, J.E. and Yeh, W.K. (1991). „Deacetoxycephalosporin C hydroxylase of Streptomyces clavuligerus. Purification, characterization, bifunctionality, and evolutionary implication”. J. Biol. Chem. 266: 5087-5093. PMID 2002049.
- ↑ Coque, J.J., Enguita, F.J., Cardoza, R.E., Martin, J.F. and Liras, P. (1996). „Characterization of the cefF gene of Nocardia lactamdurans encoding a 3′-methylcephem hydroxylase different from the 7-cephem hydroxylase”. Appl. Microbiol. Biotechnol. 44: 605-609. PMID 8703431.
- ↑ Ghag, S.K., Brems, D.N., Hassell, T.C. and Yeh, W.K. (1996). „Refolding and purification of Cephalosporium acremonium deacetoxycephalosporin C synthetase/hydroxylase from granules of recombinant Escherichia coli”. Biotechnol. Appl. Biochem. 24: 109-119. PMID 8865604.
- ↑ Lloyd, M.D., Lipscomb, S.J., Hewitson, K.S., Hensgens, C.M., Baldwin, J.E. and Schofield, C.J. (2004). „Controlling the substrate selectivity of deacetoxycephalosporin/deacetylcephalosporin C synthase”. J. Biol. Chem. 279: 15420-15426. PMID 14734549.
- ↑ Wu, X.B., Fan, K.Q., Wang, Q.H. and Yang, K.Q. (2005). „C-terminus mutations of Acremonium chrysogenum deacetoxy/deacetylcephalosporin C synthase with improved activity toward penicillin analogs”. FEMS Microbiol. Lett. 246: 103-110. PMID 15869968.
- ↑ Martín, J.F., Gutiérrez, S., Fernández, F.J., Velasco, J., Fierro, F., Marcos, A.T. and Kosalkova, K. (1994). „Expression of genes and processing of enzymes for the biosynthesis of penicillins and cephalosporins”. Antonie Van Leeuwenhoek 65: 227-243. PMID 7847890.
Literatura[uredi | uredi kod]
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.