5-epiaristolohen 1,3-dihidroksilaza
Prijeđi na navigaciju
Prijeđi na pretragu
| 5-epiaristolohen 1,3-dihidroksilaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.13.119 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
5-epiaristolohen 1,3-dihidroksilaza (EC 1.14.13.119, 5-epi-aristolohenska 1,3-dihidroksilaza, EAH) je enzim sa sistematskim imenom 5-epiaristolohen,NADPH:kiseonik oksidoreduktaza (1- i 3-hidroksilacija).[1][2] Ovaj enzim katalizuje sledeću hemijsku reakciju
- 5-epiaristolohen + 2 NADPH + 2 H+ + 2 O2 kapsidiol + 2 NADP+ + 2H2O
Ovaj enzim je hem-tiolatni protein (P-450). Kinetička istraživanja sugerišu da se uglavnom 1beta-hidroksiepiaristolohen prvo formira, i da tome sledi hidroksilacija na C-3.
Reference[uredi | uredi kod]
- ↑ Ralston, L., Kwon, S.T., Schoenbeck, M., Ralston, J., Schenk, D.J., Coates, R.M. and Chappell, J. (2001). „Cloning, heterologous expression, and functional characterization of 5-epi-aristolochene-1,3-dihydroxylase from tobacco (Nicotiana tabacum)”. Arch. Biochem. Biophys. 393: 222-235. PMID 11556809.
- ↑ Takahashi, S., Zhao, Y., O'Maille, P.E., Greenhagen, B.T., Noel, J.P., Coates, R.M. and Chappell, J. (2005). „Kinetic and molecular analysis of 5-epiaristolochene 1,3-dihydroxylase, a cytochrome P450 enzyme catalyzing successive hydroxylations of sesquiterpenes”. J. Biol. Chem. 280: 3686-3696. PMID 15522862.
Literatura[uredi | uredi kod]
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.