1,5-anhidro-D-fruktoza reduktaza (formira 1,5-anhidro-D-manitol)
| 1,5-anhidro-D-fruktoza reduktaza (formira 1,5-anhidro-D-manitol) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.1.1.292 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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1,5-anhidro-D-fruktoza reduktaza (formira 1,5-anhidro-D-manitol) (EC 1.1.1.292, 1,5-anhidro-D-fruktozna reduktaza, AFR) je enzim sa sistematskim imenom 1,5-anhidro-D-manitol:NADP+ oksidoreduktaza.[1][2] Ovaj enzim katalizuje sledeću hemijsku reakciju
- 1,5-anhidro-D-manitol + NADP+ 1,5-anhidro-D-fruktoza + NADPH + H+
Ovaj enzim je prisutan u delu Rhizobium vrsta i pripada familiji GFO/IDH/MocA proteina. On se razlikuje od hepatičke 1,5-anhidro-D-fruktozne reduktaze, koja formira 1,5-anhidro-D-glucitol kao produkt (cf. EC 1.1.1.263). Kod Sinorhizobium morelense, produkt reakcije, 1,5-anhidro-D-manitol, može da bude dalje metabolisan do D-manoze. Ovaj enzim takođe redukuje 1,5-anhidro-D-eritro-hekso-2,3-diulozu i 2-ketoaldoze, kao što je D-glukozon (D-arabino-heksoz-2-uloza) i 6-dezoksi-D-glukozon. On ne redukuje druge aldoze i ketose, niti nešećerne aldehide i ketone.
Reference[uredi | uredi kod]
- ↑ Kühn, A., Yu, S. and Giffhorn, F. (2006). „Catabolism of 1,5-anhydro-D-fructose in Sinorhizobium morelense S-30.7.5: discovery, characterization, and overexpression of a new 1,5-anhydro-D-fructose reductase and its application in sugar analysis and rare sugar synthesis”. Appl. Environ. Microbiol. 72: 1248-1257. PMID 16461673.
- ↑ Dambe, T.R., Kühn, A.M., Brossette, T., Giffhorn, F. and Scheidig, A.J. (2006). „Crystal structure of NADP(H)-dependent 1,5-anhydro-D-fructose reductase from Sinorhizobium morelense at 2.2 Å resolution: construction of a NADH-accepting mutant and its application in rare sugar synthesis”. Biochemistry 45: 10030-10042. PMID 16906761.
Literatura[uredi | uredi kod]
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- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
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